DNA barcoding reveals cryptic diversity in Lumbricus terrestris L., 1758 (Clitellata): resurrection of L. herculeus (Savigny, 1826).
DNA barcoding reveals cryptic diversity in Lumbricus terrestris L., 1758 (Clitellata): resurrection of L. herculeus (Savigny, 1826).
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DOI：
10.1371/journal.pone.0015629
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发表时间：
2010-12-29
期刊：
影响因子：
3.7
通讯作者：
Erséus C
中科院分区：
文献类型：
--
作者：
James SW;Porco D;Decaëns T;Richard B;Rougerie R;Erséus C
广泛研究的入侵性蚯蚓 Lumbricus terrestris L., 1758 多年来一直是命名争论的主题。然而，这些争议并不是基于对异质性的怀疑，而是基于与物种名称相关的描述和命名行为。获得的大量名义 L. terrestris 和 6 个同属物种的细胞色素氧化酶 I 的 DNA 条形码序列表明，名义 L. terrestris 内存在两个不同的谱系。其中一个谱系包含瑞典种群，从该种群中获得了有名的 L. terrestris 标本。另一个包含从中收集了 Enterion herculeum Savigny, 1826 的同型系列的群体。在这两种情况下，现代和古代代表都产生了条形码序列，使我们能够清楚地确定它们是两个不同的物种，与我们数据集中的任何其他同系物对一样彼此不同。除了大小相关的字符重叠之外，两者在形态上无法区分。我们已经为 L. terrestris 指定了一个新的新型。新命名的新型和大力神乳杆菌的同型产生了足以对部分细胞色素氧化酶 I 基因 (COI) 进行测序的 DNA。序列数据使得客观确定形态上与 L. terrestris 和 L. Herculeus 相同的蚯蚓的身份成为可能，无论体型大小和节数如何。过去对名义上的 L. terrestris 的研究可能是针对其中一个或两个物种，尽管在欧洲以外尚未发现 L. Herculeus。
The widely studied and invasive earthworm, Lumbricus terrestris L., 1758 has been the subject of nomenclatural debate for many years. However these disputes were not based on suspicions of heterogeneity, but rather on the descriptions and nomenclatural acts associated with the species name. Large numbers of DNA barcode sequences of the cytochrome oxidase I obtained for nominal L. terrestris and six congeneric species reveal that there are two distinct lineages within nominal L. terrestris. One of those lineages contains the Swedish population from which the name-bearing specimen of L. terrestris was obtained. The other contains the population from which the syntype series of Enterion herculeum Savigny, 1826 was collected. In both cases modern and old representatives yielded barcode sequences allowing us to clearly establish that these are two distinct species, as different from one another as any other pair of congeners in our data set. The two are morphologically indistinguishable, except by overlapping size-related characters. We have designated a new neotype for L. terrestris. The newly designated neotype and a syntype of L. herculeus yielded DNA adequate for sequencing part of the cytochrome oxidase I gene (COI). The sequence data make possible the objective determination of the identities of earthworms morphologically identical to L. terrestris and L. herculeus, regardless of body size and segment number. Past work on nominal L. terrestris could have been on either or both species, although L. herculeus has yet to be found outside of Europe.
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DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
DOI：
10.1111/j.1471-8286.2007.01678.x
发表时间：
2007-05-01
期刊：
Molecular ecology notes
影响因子：
--
作者：
Ratnasingham S;Hebert PD
通讯作者：
Hebert PD
影响因子：
4.1
作者：
Bely, AE;Wray, GA
通讯作者：
Wray, GA
影响因子：
3.7
作者：
Hale, CM;Frelich, LE;Pastor, J
通讯作者：
Pastor, J
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M