Reliability of computerized image analysis for the evaluation of serial synovial biopsies in randomized controlled trials in rheumatoid arthritis.
Reliability of computerized image analysis for the evaluation of serial synovial biopsies in randomized controlled trials in rheumatoid arthritis.
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DOI：
10.1186/ar1757
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发表时间：
2005
影响因子：
4.9
通讯作者：
Tak PP
中科院分区：
文献类型：
--
作者：
Haringman JJ;Vinkenoog M;Gerlag DM;Smeets TJ;Zwinderman AH;Tak PP
滑膜组织中生物标志物的分析越来越多地用于评估类风湿关节炎（RA）患者的新靶向治疗。本研究确定了参与临床试验的RA患者滑膜活检的数字图像分析（DIA）的内部和内部可靠性。19名RA患者参加泼尼松龙随机对照试验，在治疗前后进行关节镜滑膜活检。免疫组化检测CD3+ T细胞、CD38+浆细胞和CD68+巨噬细胞。每个标记物每平方毫米阳性细胞的平均变化由不同的操作人员在不同的时间使用DIA确定。非参数检验用于确定观察者和评估者之间的差异，并确定治疗后的变化。计算组内相关性（ICCs）以确定组内和组间的信度。肝内ICCs测量T淋巴细胞（R = 0.87）、浆细胞（R = 0.62）和巨噬细胞（R = 0.73）的变化具有良好的可靠性。Bland-Altman图分析显示，测量结果之间没有系统性差异。计算了最小的可检测变化，它们的判别能力显示，与安慰剂组相比，强的松龙组的反应良好。同样，在测量T淋巴细胞（R = 0.68）、浆细胞（R = 0.69）和巨噬细胞（R = 0.72）时，rinter - ICCs也显示出良好的可靠性。所有的测量结果都表明，与安慰剂组相比，接受治疗的患者相同的细胞类型发生了显著变化。在RA临床试验中，通过DIA测量滑膜组织中各种细胞类型的总阳性细胞数的变化可以重复地确定。
Analysis of biomarkers in synovial tissue is increasingly used in the evaluation of new targeted therapies for patients with rheumatoid arthritis (RA). This study determined the intrarater and inter-rater reliability of digital image analysis (DIA) of synovial biopsies from RA patients participating in clinical trials. Arthroscopic synovial biopsies were obtained before and after treatment from 19 RA patients participating in a randomized controlled trial with prednisolone. Immunohistochemistry was used to detect CD3+ T cells, CD38+ plasma cells and CD68+ macrophages. The mean change in positive cells per square millimetre for each marker was determined by different operators and at different times using DIA. Nonparametric tests were used to determine differences between observers and assessments, and to determine changes after treatment. The intraclass correlations (ICCs) were calculated to determine the intrarater and inter-rater reliability. Intrarater ICCs showed good reliability for measuring changes in T lymphocytes (R = 0.87), plasma cells (R = 0.62) and macrophages (R = 0.73). Analysis by Bland–Altman plots showed no systemic differences between measurements. The smallest detectable changes were calculated and their discriminatory power revealed good response in the prednisolone group compared with the placebo group. Similarly, inter-rater ICCs also revealed good reliability for measuring T lymphocytes (R = 0.68), plasma cells (R = 0.69) and macrophages (R = 0.72). All measurements identified the same cell types as changing significantly in the treated patients compared with the placebo group. The measurement of change in total positive cell numbers in synovial tissue can be determined reproducibly for various cell types by DIA in RA clinical trials.
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影响因子：
2.2
作者：
NORAZMI, MN;HOHMANN, AW;BRADLEY, J
通讯作者：
BRADLEY, J
影响因子：
--
作者：
Gerlag, DM;Haringman, JJ;Tak, PP
通讯作者：
Tak, PP
影响因子：
--
作者：
Smeets, TJM;Kraan, MC;Tak, PP
通讯作者：
Tak, PP
影响因子：
27.4
作者：
Kraan, MC;Smith, MD;Tak, PP
通讯作者：
Tak, PP
影响因子：
--
作者：
Kraan, MC;Reece, RJ;Tak, PP
通讯作者：
Tak, PP