A conserved stem of the Myxococcus xanthus sRNA Pxr controls sRNA accumulation and multicellular development.
A conserved stem of the Myxococcus xanthus sRNA Pxr controls sRNA accumulation and multicellular development.
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DOI：
10.1038/s41598-017-15439-w
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发表时间：
2017-11-13
影响因子：
4.6
通讯作者：
Velicer GJ
中科院分区：
文献类型：
--
作者：
Yu YN;Cooper E;Velicer GJ
小RNA（sRNA）Pxr负控制细菌粘球菌（Myxococcus xanthus）中多细胞子实体的形成，在营养丰富时抑制从生长到发育的转变。与许多其他原核sRNA一样，Pxr被预测折叠成三个茎环（SL 1-SL 3）。SL 1和SL 2在粘细菌中是高度保守的，而SL 3则变化更大。SL 1对于Pxr的调节功能是必需的，但SL 3在这方面的重要性尚不清楚。为了测试Pxr功能所需的顺式遗传元件，我们从发育缺陷菌株中删除了整个pxr基因，该菌株未能消除Pxr介导的发育阻断，并重新引入pxr区域的截短片段以测试其阻断发育的能力。这些截短证明了SL 3对于缺陷菌株中的Pxr功能是必需的。我们进一步表明，一个高度保守的8个碱基对的SL 3片段不仅是必要的Pxr在饥饿条件下的缺陷菌株中阻止发展，但也需要Pxr，以防止子实体的发展，在高营养条件下的发育熟练的野生型菌株。SL 3的这一保守片段也是可检测水平的Pxr积累所必需的，这表明该片段在营养生长期间稳定Pxr以防止过早降解或正调控其转录。
The small RNA (sRNA) Pxr negatively controls multicellular fruiting body formation in the bacterium Myxococcus xanthus, inhibiting the transition from growth to development when nutrients are abundant. Like many other prokaryotic sRNAs, Pxr is predicted to fold into three stem loops (SL1-SL3). SL1 and SL2 are highly conserved across the myxobacteria, whereas SL3 is much more variable. SL1 is necessary for the regulatory function of Pxr but the importance of SL3 in this regard is unknown. To test for cis genetic elements required for Pxr function, we deleted the entire pxr gene from a developmentally defective strain that fails to remove Pxr-mediated blockage of development and reintroduced variably truncated fragments of the pxr region to test for their ability to block development. These truncations demonstrated that SL3 is necessary for Pxr function in the defective strain. We further show that a highly conserved eight-base-pair segment of SL3 is not only necessary for Pxr to block development in the defective strain under starvation conditions, but is also required for Pxr to prevent fruiting body development by a developmentally proficient wild-type strain under high-nutrient conditions. This conserved segment of SL3 is also necessary for detectable levels of Pxr to accumulate, suggesting that this segment either stabilizes Pxr against premature degradation during vegetative growth or positively regulates its transcription.
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影响因子：
14.9
作者：
Guillier, Maude;Gottesman, Susan
通讯作者：
Gottesman, Susan
影响因子：
4.1
作者：
Chen, I-Chen Kimberly;Griesenauer, Brad;Velicer, Gregory J.
通讯作者：
Velicer, Gregory J.
DOI：
10.1073/pnas.95.21.12376
发表时间：
1998-10-13
影响因子：
11.1
作者：
Velicer, GJ;Kroos, L;Lenski, RE
通讯作者：
Lenski, RE
DOI：
10.1073/pnas.0510740103
发表时间：
2006-05-23
影响因子：
11.1
作者：
Velicer, Gregory J.;Raddatz, Guenter;Schuster, Stephan C.
通讯作者：
Schuster, Stephan C.
DOI：
10.1126/science.1187200
发表时间：
2010-05-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Yu YT;Yuan X;Velicer GJ
通讯作者：
Velicer GJ