Application of a qPCR assay in the investigation of susceptibility to malaria infection of the M and S molecular forms of An. gambiae s.s. in Cameroon.
Application of a qPCR assay in the investigation of susceptibility to malaria infection of the M and S molecular forms of An. gambiae s.s. in Cameroon.
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DOI：
10.1371/journal.pone.0054820
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Morlais I
中科院分区：
文献类型：
--
作者：
Boissière A;Gimonneau G;Tchioffo MT;Abate L;Bayibeki A;Awono-Ambéné PH;Nsango SE;Morlais I
恶性疟原虫是疟疾的病原体，疟疾每年导致近100万人死亡，主要发生在撒哈拉以南非洲。恶性疟原虫通过按蚊雌蚊的叮咬传播给人类宿主，冈比亚按蚊是非洲最大的疟疾媒介，广泛分布于非洲热带地区。一种。冈比亚亚种分为两种不同的分子形式，即M型和S型。这两种分子形式在形态上是相同的，但它们在遗传上是不同的，并且它们的分布和生态偏好不同。到目前为止，这两种分子形式在疟疾传播中的流行病学重要性一直没有得到很好的调查，并在不同地区得出了不同的结果。我们建立了一种实时荧光定量聚合酶链式反应(QPCR)方法，并用于检测野生按蚊卵囊期恶性疟原虫。冈比亚亚种实验感染了自然寄生虫分离物的蚊子。蚊子在同域和异地繁殖点采集幼虫，成虫后进一步感染。接下来，我们使用qPCR方法测量了雌性蚊子的感染流行率和感染强度，并将感染成功率与蚊子的分子形式相关联。我们的结果显示，M和S两种分子形式的AN的感染率不同。冈比亚亚种在喀麦隆，对于同域和异地的蚊子种群都是如此。但感染强度无明显差异。因此，AN的分子形式的分布。冈比亚亚种可能会对疟疾流行病学产生影响，因此监测M和S两种疟疾防治干预措施的效果将非常重要。
Plasmodium falciparum is the causative agent of malaria, a disease that kills almost one million persons each year, mainly in sub-Saharan Africa. P. falciparum is transmitted to the human host by the bite of an Anopheles female mosquito, and Anopheles gambiae sensus stricto is the most tremendous malaria vector in Africa, widespread throughout the afro-tropical belt. An. gambiae s.s. is subdivided into two distinct molecular forms, namely M and S forms. The two molecular forms are morphologically identical but they are distinct genetically, and differ by their distribution and their ecological preferences. The epidemiological importance of the two molecular forms in malaria transmission has been poorly investigated so far and gave distinct results in different areas. We have developed a real-time quantitative PCR (qPCR) assay, and used it to detect P. falciparum at the oocyst stage in wild An. gambiae s.s. mosquitoes experimentally infected with natural isolates of parasites. Mosquitoes were collected at immature stages in sympatric and allopatric breeding sites and further infected at the adult stage. We next measured the infection prevalence and intensity in female mosquitoes using the qPCR assay and correlated the infection success with the mosquito molecular forms. Our results revealed different prevalence of infection between the M and S molecular forms of An. gambiae s.s. in Cameroon, for both sympatric and allopatric populations of mosquitoes. However, no difference in the infection intensity was observed. Thus, the distribution of the molecular forms of An. gambiae s.s. may impact on the malaria epidemiology, and it will be important to monitor the efficiency of malaria control interventions on the two M and S forms.
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影响因子：
4
作者：
Bell, AS;Ranford-Cartwright, LC
通讯作者：
Ranford-Cartwright, LC
影响因子：
2.9
作者：
Berry, A.;Benoit-Vical, F.;Magnaval, J. F.
通讯作者：
Magnaval, J. F.
DOI：
10.1016/0035-9203(80)90189-3
发表时间：
1980-01-01
影响因子：
2.2
作者：
GRAVES, PM
通讯作者：
GRAVES, PM
影响因子：
2.4
作者：
Fabre, R;Berry, A;Magnaval, JF
通讯作者：
Magnaval, JF
影响因子：
15.8
作者：
Alonso PL;Brown G;Arevalo-Herrera M;Binka F;Chitnis C;Collins F;Doumbo OK;Greenwood B;Hall BF;Levine MM;Mendis K;Newman RD;Plowe CV;Rodríguez MH;Sinden R;Slutsker L;Tanner M
通讯作者：
Tanner M