Development of an odorant sensor with a cell-free synthesized olfactory receptor and a graphene field-effect transistor
Development of an odorant sensor with a cell-free synthesized olfactory receptor and a graphene field-effect transistor
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开发具有无细胞合成嗅觉受体和石墨烯场效应晶体管的气味传感器
DOI：
10.1007/s44211-022-00073-y
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发表时间：
2022
影响因子：
1.6
通讯作者：
Yohda Masafumi
中科院分区：
文献类型：
--
作者：
Yoshii Tomoya;Takayama Ikumi;Fukutani Yosuke;Ikuta Takashi;Maehashi Kenzo;Yohda Masafumi
动物通过嗅觉感受器感知气味。已经进行了许多试验来开发使用嗅觉受体的人工气味传感器。然而，由于嗅觉受体难以获得，阻碍了其发展。在这项研究中，我们表达了嗅觉受体，cOR 52，使用麦胚无细胞合成系统。通过与mini-G蛋白的配体浓度依赖性相互作用证实了表达的cOR 52的功能性。将表达的cOR 52固定在石墨烯场效应晶体管上。cOR 52修饰的石墨烯场效应晶体管在100 nM和100 µM之间表现出配体特异性响应。这种方法似乎适用于其他嗅觉受体。因此，通过该方法，可以开发具备各种嗅觉感受器的气味传感器。
Animals sense odorants using olfactory receptors. Many trials have been conducted to develop artificial odorant sensors using olfactory receptors. However, the development has been hindered by the difficulty in obtaining olfactory receptors. In this study, we expressed an olfactory receptor, cOR52, using a wheat germ cell-free synthesis system. The functionality of the expressed cOR52 was confirmed by ligand concentration-dependent interactions with the mini-G protein. The expressed cOR52 was immobilized on a graphene field-effect transistor. The cOR52-modified graphene field-effect transistor exhibited a ligand-specific response between 100 nM and 100 µM. This approach seems to be applicable for other olfactory receptors. Therefore, it will be possible to develop an odorant sensor equipped with various olfactory receptors by this method.Graphical abstract
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DOI：
10.1073/pnas.0307882100
发表时间：
2004-02-24
影响因子：
11.1
作者：
Malnic, B;Godfrey, PA;Buck, LB
通讯作者：
Buck, LB
影响因子：
5.6
作者：
Suzuki Y;Ogasawara T;Tanaka Y;Takeda H;Sawasaki T;Mogi M;Liu S;Maeyama K
通讯作者：
Maeyama K
影响因子：
4.2
作者：
Goodwin, Danielle M.;Walters, Ffion;Guy, Owen J.
通讯作者：
Guy, Owen J.
影响因子：
64.5
作者：
Krautwurst, D;Yau, KW;Reed, RR
通讯作者：
Reed, RR