Expression analysis of limb element markers during mouse embryonic development.
Expression analysis of limb element markers during mouse embryonic development.
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DOI：
10.1002/dvdy.24671
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发表时间：
2018-11
期刊：
影响因子：
--
通讯作者：
Vargesson N
中科院分区：
文献类型：
--
作者：
Rafipay A;Berg ALR;Erskine L;Vargesson N
背景资料：虽然存在关于正常小鼠肢体发育期间肢体元件和组织标记物表达的数据，但很少有研究显示在整个关键肢体发育阶段的上肢和下肢中的表达模式。当分析突变小鼠模型中肢体的发育时，与正常发育事件的比较是必不可少的。结果如下：关节标志物Gdf 5、肌腱和韧带标志物Scleraxis、早期肌肉标志物MyoD 1和血管标志物钙粘蛋白5（Cdh 5）的表达模式在胚胎小鼠肢体图案形成的最活跃阶段呈现。还描述了前肢和后肢中发育神经的抗神经丝染色以及软骨形成和进展。结论：这项研究展示并描述了一系列关键的形态学标记和方法，这些标记和方法可以一起用于评估正常和异常的肢体发育。发展动力学247：1217-1226，2018。版权所有2018作者。出版社：Wiley Periodicals，Inc.代表美国解剖学家协会在整个前肢和后肢发育过程中的分子标记物表达模式-可用于评估正常和异常发育。详细描述了前肢和后肢发育过程中的神经支配，证实了在肢体图案形成事件开始后首次观察到的神经支配。软骨发育和进展的描述表明，直到E15.5才在前肢和后肢中观察到茜素红染色。后肢在分子和形态上落后于前肢，直到E14.5。详细描述了用于研究前肢和后肢发育的方法。
Background: While data regarding expression of limb element and tissue markers during normal mouse limb development exist, few studies show expression patterns in upper and lower limbs throughout key limb development stages. A comparison to normal developmental events is essential when analyzing development of the limb in mutant mice models. Results: Expression patterns of the joint marker Gdf5, tendon and ligament marker Scleraxis, early muscle marker MyoD1, and blood vessel marker Cadherin5 (Cdh5) are presented during the most active phases of embryonic mouse limb patterning. Anti‐neurofilament staining of developing nerves in the fore‐ and hindlimbs and cartilage formation and progression also are described. Conclusions: This study demonstrates and describes a range of key morphological markers and methods that together can be used to assess normal and abnormal limb development. Developmental Dynamics 247:1217–1226, 2018. © 2018 The Authors. Developmental Dynamics published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists Expression patterns of molecular markers throughout both fore‐ and hindlimb development ‐ which can be used to assess normal and abnormal development. Detailled description of innervation during fore‐ and hindlimb development confirming innervation first seen after limb patterning events have begun. Description of cartilage development and progression indicates alizarin red staining not seen until E15.5 in both fore‐ and hindlimbs. Hindlimb lags behind forelimb molecularly and morphologically until E14.5. Detailled description of methods used to study fore‐ and hindlimb development.
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