Interstitial cells of Cajal mediate excitatory sympathetic neurotransmission in guinea pig prostate
Interstitial cells of Cajal mediate excitatory sympathetic neurotransmission in guinea pig prostate
复制标题
卡哈尔间质细胞介导豚鼠前列腺兴奋性交感神经传递
DOI：
10.1007/s00441-013-1572-3
复制
发表时间：
2013-02
影响因子：
3.6
通讯作者：
Wang, Qin-zhang
中科院分区：
文献类型：
--
作者：
Wang, Jiang-ping;Ding, Guo-fu;Wang, Qin-zhang
形态学和功能研究证实，Cajal间质细胞（ICCs）参与多种肠道运动神经传递途径。最近的研究表明，人类和豚鼠前列腺具有独特的细胞类型，与ICC在形态学和免疫学上具有相似性。这些前列腺ICC与神经束和平滑肌细胞有密切的关系。前列腺平滑肌张力主要由交感神经系统的刺激引起，交感神经系统释放兴奋性去甲肾上腺素（NE）作用于α1-肾上腺素受体。我们已经进行了形态学和功能实验，以确定在豚鼠前列腺的交感神经传递的基础上的假设，前列腺ICCs作为交感神经传递介质的ICCs的作用。免疫组化显示ICC与交感神经束和平滑肌细胞之间有许多紧密的接触点。双标记切片显示前列腺ICC中表达α1肾上腺素受体和差距连接蛋白连接蛋白43。令人惊讶的是，前列腺ICC共表达酪氨酸羟化酶和多巴胺β-羟化酶，这是交感神经元的两种标志物。在功能上，NE的应用引起了一个大的单一内向电流在离体前列腺ICC的剂量依赖性的方式。NE诱发的内向电流是通过激活α1肾上腺素受体介导的，因为它被非特异性α 1肾上腺素受体拮抗剂酚妥拉明和特异性α1肾上腺素受体拮抗剂哌唑嗪所阻断。因此，豚鼠前列腺中的ICC是前列腺交感神经的靶细胞，并且具有介导交感信号所需的形态学和功能特征。
Morphological and functional studies have confirmed that interstitial cells of Cajal (ICCs) are involved in many enteric motor neurotransmission pathways. Recent investigations have demonstrated that human and guinea pig prostate glands possess a distinct cell type with morphological and immunological similarities to ICCs. These prostate ICCs have a close relationship with nerve bundles and smooth muscle cells. Prostate smooth muscle tone is largely induced by stimulation from the sympathetic nervous system, which releases excitatory norepinephrine (NE) to act on the α1-adrenoceptor. We have performed morphological and functional experiments to determine the role of ICCs in sympathetic neurotransmission in the guinea pig prostate based on the hypothesis that prostate ICCs act as mediators of sympathetic neurotransmission. Immunohistochemistry revealed many close points of contact between ICCs and sympathetic nerve bundles and smooth muscle cells. Double-labeled sections revealed that α1-adrenoceptor and the gap junction protein connexin 43 were expressed in prostate ICCs. Surprisingly, prostate ICCs co-expressed tyrosine hydroxylase and dopamine β-hydroxylase, two markers of sympathetic neurons. Functionally, the application of NE evoked a large single inward current in isolated prostate ICCs in a dose-dependent manner. The inward current evoked by NE was mediated via the activation of α1-adrenoceptors, because it was abolished by the non-specific α-adrenoceptor antagonist, phentolamine and the specific α1-adrenoceptor antagonist, prazosin. Thus, ICCs in the guinea pig prostate are target cells for prostate sympathetic nerves and possess the morphological and functional characteristics required to mediate sympathetic signals.
登录
查看更多内容
影响因子：
5
作者：
Jun, JY;Choi, S;Kim, KW
通讯作者：
Kim, KW
影响因子：
3.5
作者：
S. Ward;Kenton M. Sanders;G. Hirst
通讯作者：
S. Ward;Kenton M. Sanders;G. Hirst
影响因子：
2.5
作者：
Beckett, EAH;Takeda, Y;Ward, SM
通讯作者：
Ward, SM
影响因子：
3.6
作者：
H. Salmhofer;W. Neuhuber;P. Ruth;A. Huber;M. Russwurm;H. Allescher
通讯作者：
H. Salmhofer;W. Neuhuber;P. Ruth;A. Huber;M. Russwurm;H. Allescher
影响因子：
4.6
作者：
Maria M. Picken
通讯作者：
Maria M. Picken