Characterization of major histocompatibility complex-related molecule 1 sequence variants in non-human primates.
Characterization of major histocompatibility complex-related molecule 1 sequence variants in non-human primates.
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DOI：
10.1007/s00251-018-1091-1
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发表时间：
2019-03
期刊：
影响因子：
3.2
通讯作者：
O'Connor SL
中科院分区：
文献类型：
--
作者：
Ellis-Connell AL;Kannal NM;Balgeman AJ;O'Connor SL
The Major Histocompatibility complex (MHC) class I-related molecule, MR1, presents vitamin B metabolites from bacteria and yeast to Mucosal-associated invariant T (MAIT) cells. Despite the evolutionary conservation of MR1, we do not know whether different allele variants of MR1 exist within the nonhuman primate (NHP) populations that are commonly used for biomedical research. In this study, we identified 21 distinct MR1 nucleotide sequences representing 32 different alleles across five different NHP populations. The majority of the alleles conferring amino acid changes (allele variants) were found in or near the alpha-1 domain of the mature MR1 protein. We expressed four of the most commonly observed MR1 allele variants in 293T cells, and we found that each variant could present bacterial metabolites on the cell surface. We successfully induced cytokine production in macaque MAIT cells stimulated with 293T cells expressing the four most common MR1 allele variants, demonstrating the usefulness of these cell lines to study MAIT cell activity. Our data suggests that MR1 is not monomorphic, but that there are multiple MR1 alleles in NHPs. The materials we describe here will be valuable for characterizing differences in MR1 antigen presentation and MAIT cell function in NHPs.
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影响因子：
56.9
作者：
HASHIMOTO, K;HIRAI, M;KUROSAWA, Y
通讯作者：
KUROSAWA, Y
影响因子：
30.5
作者：
Le Bourhis, Lionel;Martin, Emmanuel;Lantz, Olivier
通讯作者：
Lantz, Olivier
影响因子：
6.7
作者：
Le Bourhis L;Dusseaux M;Bohineust A;Bessoles S;Martin E;Premel V;Coré M;Sleurs D;Serriari NE;Treiner E;Hivroz C;Sansonetti P;Gougeon ML;Soudais C;Lantz O
通讯作者：
Lantz O
DOI：
10.1073/pnas.1222678110
发表时间：
2013-05-07
影响因子：
11.1
作者：
Lopez-Sagaseta, Jacinto;Dulberger, Charles L.;Adams, Erin J.
通讯作者：
Adams, Erin J.
影响因子：
9.8
作者：
Gold MC;Cerri S;Smyk-Pearson S;Cansler ME;Vogt TM;Delepine J;Winata E;Swarbrick GM;Chua WJ;Yu YY;Lantz O;Cook MS;Null MD;Jacoby DB;Harriff MJ;Lewinsohn DA;Hansen TH;Lewinsohn DM
通讯作者：
Lewinsohn DM