Spatiotemporal organization of cilia drives multiscale mucus swirls in model human bronchial epithelium.
Spatiotemporal organization of cilia drives multiscale mucus swirls in model human bronchial epithelium.
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DOI：
10.1038/s41598-018-20882-4
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发表时间：
2018-02-05
影响因子：
4.6
通讯作者：
Viallat A
中科院分区：
文献类型：
--
作者：
Khelloufi MK;Loiseau E;Jaeger M;Molinari N;Chanez P;Gras D;Viallat A
黏膜纤毛清除是气道保护的生物力学机制。它包括粘液沿着支气管树的主动运输，粘液是由呼吸道上皮表面上无数纤毛的协调跳动推动的流体。粘液运输的物理学知之甚少，因为它涉及复杂的现象，如长程流体动力学相互作用，主动集体纤毛运动，以及粘液的复杂流变学。我们提出了一个定量物理分析的纤毛活动和粘液运输的一大面板的人支气管文化从对照组，哮喘和慢性阻塞性肺疾病患者从支气管内活检。在这里，我们报告存在多个纤毛域的大小范围从几十微米到厘米，纤毛节拍呈现圆形取向顺序。这些结构域与环形粘液流动模式相关，其大小与平均纤毛密度成比例。在这些领域中，我们发现，纤毛细胞密度的径向增加加上纤毛节拍的取向顺序的增加，导致在一个净本地力成比例的粘液速度。我们提出了一个现象学的物理模型，支持我们的结果。
Mucociliary clearance is a biomechanical mechanism of airway protection. It consists of the active transport along the bronchial tree of the mucus, a fluid propelled by the coordinated beating of a myriad of cilia on the epithelial surface of the respiratory tract. The physics of mucus transport is poorly understood because it involves complex phenomena such as long-range hydrodynamic interactions, active collective ciliary motion, and the complex rheology of mucus. We propose a quantitative physical analysis of the ciliary activity and mucus transport on a large panel of human bronchial cultures from control subjects, patients with asthma and chronic obstructive pulmonary disease obtained from endobronchial biopsies. Here we report on the existence of multiple ciliary domains with sizes ranging from the tens of a micron to the centimeter, where ciliary beats present a circular orientational order. These domains are associated with circular mucus flow patterns, whose size scales with the average cilia density. In these domains, we find that the radial increase of the ciliated cell density coupled with the increase in the orientational order of ciliary beats result in a net local force proportional to the mucus velocity. We propose a phenomenological physical model that supports our results.
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DOI：
10.1126/science.1223012
发表时间：
2012-08-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Button B;Cai LH;Ehre C;Kesimer M;Hill DB;Sheehan JK;Boucher RC;Rubinstein M
通讯作者：
Rubinstein M
影响因子：
56.9
作者：
Faubel, Regina;Westendorf, Christian;Eichele, Gregor
通讯作者：
Eichele, Gregor
影响因子：
3.3
作者：
Foster, WM;Walters, DM;Miller, LM
通讯作者：
Miller, LM
影响因子：
64.8
作者：
Lafkas, Daniel;Shelton, Amy;Siebel, Christian W.
通讯作者：
Siebel, Christian W.
影响因子：
44.1
作者：
Marchetti, M. C.;Joanny, J. F.;Simha, R. Aditi
通讯作者：
Simha, R. Aditi