Differential regulation of tissue factor and plasminogen activator inhibitor by human mononuclear cells
Differential regulation of tissue factor and plasminogen activator inhibitor by human mononuclear cells
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人单核细胞对组织因子和纤溶酶原激活剂抑制剂的差异调节
DOI：
10.1182/blood.v74.5.1644.bloodjournal7451644
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发表时间：
1989
期刊：
影响因子：
20.3
通讯作者：
J. Bradshaw
中科院分区：
文献类型：
--
作者：
B. Schwartz;J. Bradshaw
炎症是免疫介导的组织病变的标志。这种病变中纤维蛋白的存在意味着通过凝血形成纤维蛋白和伴随的纤维蛋白溶解的限制，从而使纤维蛋白持续存在。以前的工作表明，暴露于炎症刺激物如脂多糖（LPS）的人单核细胞产生组织因子（TF）和纤溶酶原激活物抑制剂2型（派-2）。这两种蛋白质有利于纤维蛋白沉积，有证据表明这两种分子的细胞产生可能是相关的。另一个与免疫介导的组织损伤和纤维蛋白沉积有关的促炎过程是对同种异体抗原的应答。由淋巴细胞和单核细胞组成的外周血单个核细胞（PBM）对同种异体抗原刺激有不同的TF和派-2表达。PBM暴露于同种异体抗原开发高水平的TF活性，派-2活性或抗原没有伴随增加。同种异体抗原刺激的PBM不积累细胞内派-2，也不降解派-2添加到培养物中。这种派-2生产的缺乏是由于刺激不足，氚标记的胸苷摄取和TF生产证明了识别，并强烈反应，同种异体抗原。PBM暴露于同种异体抗原的TF和派-2反应的差异维持超过5天，并通过mRNA谱反映。这些结果表明，在特定的生理相关条件下，炎症单核细胞的促凝血和抗纤溶效应可以独立调节。这意味着单核细胞机制比以前认为的更有利于纤维蛋白沉积。
Fibrin is a hallmark of immune-mediated tissue lesions. The presence of fibrin in such lesions implies both the formation of fibrin via coagulation and the accompanying restriction of fibrinolysis, allowing fibrin to persist. Previous work has shown that human monocytes exposed to an inflammatory stimulus such as lipopolysaccharide (LPS) produce both tissue factor (TF) and plasminogen activator inhibitor--type 2 (PAI-2). These two proteins favor fibrin deposition, and evidence implies that cellular production of these two molecules may be linked. Another proinflammatory process pertinent to immune-mediated tissue damage and fibrin deposition is the response to alloantigen. Peripheral- blood mononuclear cells (PBM), consisting of lymphocytes and monocytes together, responded to alloantigen stimulation with differential expression of TF and PAI-2. PBM exposed to alloantigen developed high levels of TF activity, with no concomitant increase in PAI-2 activity or antigen. Alloantigen-stimulated PBM did not accumulate intracellular PAI-2, nor did they degrade PAI-2 added to cultures. This lack of PAI-2 production was not due to inadequate stimulation, as tritiated thymidine uptake and TF production demonstrated recognition of, and a vigorous reaction to, alloantigen. The divergent TF and PAI-2 responses of PBM exposed to alloantigen was maintained over 5 days and was reflected by mRNA profiles. These results imply that under specific physiologically relevant conditions, the procoagulant and antifibrinolytic effectors of inflammatory mononuclear cells can be independently regulated. This would imply more flexibility to monocyte mechanisms that favor fibrin deposition than previously thought.
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组织因子（因子 VII 的高亲和力细胞受体）的单体和异二聚体形式的解析。
影响因子：
7.5
作者：
Morrissey,JH;Revak,D;Tejada,P;Fair,DS;Edgington,TS
通讯作者：
Edgington,TS
细菌脂多糖直接淋巴细胞诱导人促凝血单核因子的动力学和代谢要求。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Levy,GA;Schwartz,BS;Edgington,TS
通讯作者：
Edgington,TS
2 型纤溶酶原激活剂抑制剂的释放增加伴随着人类单核细胞组织因子对脂多糖的反应。
影响因子：
20.3
作者：
Schwartz,BS;Monroe,MC;Levin,EG
通讯作者：
Levin,EG
人血小板聚集是由体外暴露于细菌脂多糖的外周血单核细胞引发的。
DOI：
10.1172/jci112693
发表时间：
1986
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Schwartz,BS;Monroe,MC
通讯作者：
Monroe,MC
人体组织因子：基因的 cDNA 序列和染色体定位。
影响因子：
2.9
作者：
Scarpati,EM;Wen,D;BrozeJr,GJ;Miletich,JP;Flandermeyer,RR;Siegel,NR;Sadler,JE
通讯作者：
Sadler,JE