The maternal transcriptome of the crustacean Parhyale hawaiensis is inherited asymmetrically to invariant cell lineages of the ectoderm and mesoderm.
The maternal transcriptome of the crustacean Parhyale hawaiensis is inherited asymmetrically to invariant cell lineages of the ectoderm and mesoderm.
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DOI：
10.1371/journal.pone.0056049
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gerberding M
中科院分区：
文献类型：
--
作者：
Nestorov P;Battke F;Levesque MP;Gerberding M
夏威夷甲壳动物的胚胎具有完全的、不平等的和不变的早期卵裂模式。它比包括果蝇在内的其他节肢动物更早指定细胞命运，因为8细胞阶段的单个卵裂球被分配给生殖层和生殖系。此外，8-细胞期可以接受胚胎学操作。这些独特的特征使Parhyale成为一种适合于阐明节肢动物生殖层规格的系统。由于母体提供的RNA的不对称定位是决定早期细胞命运的一种广泛的机制，我们询问这是否也适用于Parhyale。一种候选基因方法没有发现在8细胞阶段不对称分布的RNA。因此，我们设计了一个高密度的微阵列，从9400个最近测序的EST中(1)识别母体提供的RNA，(2)寻找在8-细胞期细胞中差异分布的RNA。母体-受精卵的转变发生在32-细胞期左右，即在生殖层指定之后。通过比较来自没有合子转录的早期胚胎的RNA池和生殖带的合子RNA池，我们发现阵列上超过10%的靶子在母体转录库中富含。对8-细胞期不对称分布的RNA进行筛选，发现129个转录本，其中50%主要在早期胚胎阶段表达。最后，我们对其中两个基因进行了敲除实验，并观察到了胚胎发育中与细胞命运相关的缺陷。与果蝇不同，Parhyale的四个初级生殖层细胞谱系是在胚胎的母体控制阶段指定的。在这一过程中的一个关键步骤是大量母体RNA不对称地分布到生殖层祖细胞。
The embryo of the crustacean Parhyale hawaiensis has a total, unequal and invariant early cleavage pattern. It specifies cell fates earlier than other arthropods, including Drosophila, as individual blastomeres of the 8-cell stage are allocated to the germ layers and the germline. Furthermore, the 8-cell stage is amenable to embryological manipulations. These unique features make Parhyale a suitable system for elucidating germ layer specification in arthropods. Since asymmetric localization of maternally provided RNA is a widespread mechanism to specify early cell fates, we asked whether this is also true for Parhyale. A candidate gene approach did not find RNAs that are asymmetrically distributed at the 8-cell stage. Therefore, we designed a high-density microarray from 9400 recently sequenced ESTs (1) to identify maternally provided RNAs and (2) to find RNAs that are differentially distributed among cells of the 8-cell stage. Maternal-zygotic transition takes place around the 32-cell stage, i.e. after the specification of germ layers. By comparing a pool of RNAs from early embryos without zygotic transcription to zygotic RNAs of the germband, we found that more than 10% of the targets on the array were enriched in the maternal transcript pool. A screen for asymmetrically distributed RNAs at the 8-cell stage revealed 129 transcripts, from which 50% are predominantly expressed in the early embryonic stages. Finally, we performed knockdown experiments for two of these genes and observed cell-fate-related defects of embryonic development. In contrast to Drosophila, the four primary germ layer cell lineages in Parhyale are specified during the maternal control phase of the embryo. A key step in this process is the asymmetric distribution of a large number of maternal RNAs to the germ layer progenitor cells.
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DOI：
10.1126/science.1197761
发表时间：
2011-02-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colbourne JK;Pfrender ME;Gilbert D;Thomas WK;Tucker A;Oakley TH;Tokishita S;Aerts A;Arnold GJ;Basu MK;Bauer DJ;Cáceres CE;Carmel L;Casola C;Choi JH;Detter JC;Dong Q;Dusheyko S;Eads BD;Fröhlich T;Geiler-Samerotte KA;Gerlach D;Hatcher P;Jogdeo S;Krijgsveld J;Kriventseva EV;Kültz D;Laforsch C;Lindquist E;Lopez J;Manak JR;Muller J;Pangilinan J;Patwardhan RP;Pitluck S;Pritham EJ;Rechtsteiner A;Rho M;Rogozin IB;Sakarya O;Salamov A;Schaack S;Shapiro H;Shiga Y;Skalitzky C;Smith Z;Souvorov A;Sung W;Tang Z;Tsuchiya D;Tu H;Vos H;Wang M;Wolf YI;Yamagata H;Yamada T;Ye Y;Shaw JR;Andrews J;Crease TJ;Tang H;Lucas SM;Robertson HM;Bork P;Koonin EV;Zdobnov EM;Grigoriev IV;Lynch M;Boore JL
通讯作者：
Boore JL
影响因子：
64.5
作者：
Lecuyer, Eric;Yoshida, Hideki;Krause, Henry M.
通讯作者：
Krause, Henry M.
影响因子：
2.9
作者：
Lorenzini, DM;da Silva, PI;Daffre, S
通讯作者：
Daffre, S
对夏威夷 Parhyale mRNA 和 microRNA 进行高通量测序，以帮助比较发育研究。
影响因子：
3.7
作者：
Blythe MJ;Malla S;Everall R;Shih YH;Lemay V;Moreton J;Wilson R;Aboobaker AA
通讯作者：
Aboobaker AA
影响因子：
3
作者：
Battke F;Symons S;Nieselt K
通讯作者：
Nieselt K