Comparative study of Clostridium difficile toxin A and cholera toxin in rabbit ileum.
Comparative study of Clostridium difficile toxin A and cholera toxin in rabbit ileum.
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兔回肠中艰难梭菌毒素A与霍乱毒素的比较研究。
DOI：
10.1016/0016-5085(89)91689-2
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发表时间：
1989
期刊：
影响因子：
29.4
通讯作者：
Lamont,JT
中科院分区：
文献类型：
--
作者：
Triadafilopoulos,G;Pothoulakis,C;Weiss,R;Giampaolo,C;Lamont,JT
本研究的目的是比较艰难梭菌毒素A和霍乱毒素对家兔回肠液体分泌、肠通透性和花生四烯酸代谢产物的影响。将25 μg纯化的毒素注射到10 cm回肠袢中，引起液体分泌和肠对甘露醇的渗透性以及前列腺素E2释放到管腔中的显著增加。毒素A，但不是霍乱毒素，引起严重的固有层炎症反应和肠细胞坏死，以及增加释放的白三烯B4。毒素A介导的前列腺素E2和白三烯B4的增加可以通过预先向回肠袢滴注10 mg 5-氨基水杨酸来阻断。5-氨基水杨酸也显着减少了预期的增加甘露醇的渗透性后，两种毒素，但没有显着的抑制作用，液体分泌或毒素A的情况下，肠道炎症。结果表明，C.艰难梭菌毒素A是一种炎性毒素，它能引起中性粒细胞对固有层的显著浸润，这与白三烯B4的释放增加有关。相反，霍乱毒素不会引起炎症或释放白三烯B4。暴露于两种毒素后，前列腺素E2的释放增加，似乎与肠道通透性增加有关。
The purpose of this study was to compare the effects ofClostridium difficiletoxin A and cholera toxin on fluid secretion, intestinal permeability, and arachidonate metabolites in rabbit ileum. Injection of 25 μg of either purified toxin into 10-cm ileal loops caused significant increases in fluid secretion and intestinal permeability to mannitol as well as release of prostaglandin E2into the lumen. Toxin A, but not cholera toxin, caused a severe inflammatory reaction of the lamina propria and necrosis of enterocytes as well as increased release of leukotriene B4. The toxin A-mediated increases in prostaglandin E2and leukotriene B4could be blocked by prior instillation of 10 mg of 5-aminosalicylic acid into ileal loops. 5-Aminosalicylic acid also significantly diminished the expected increase in mannitol permeability after both toxins, but had no significant inhibitory effect on fluid secretion or, in the case of toxin A, intestinal inflammation. Our results indicate thatC. difficileand cholera enterotoxins differ substantially in their effects on the rabbit intestine.Clostridium difficiletoxin A, an inflammatory toxin, produces a striking infiltration of the lamina propria with neutrophils that is associated with increased release of leukotriene B4. In contrast, cholera toxin does not cause inflammation or leukotriene B4release. Increased release of prostaglandin E2occurs after exposure to both toxins and appears to be correlated with increased intestinal permeability.
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艰难梭菌毒素 A 和 B 对兔回肠的不同影响。
影响因子：
29.4
作者：
Triadafilopoulos,G;Pothoulakis,C;O'Brien,MJ;LaMont,JT
通讯作者：
LaMont,JT
肉毒杆菌C2毒素对小鼠肠道的组织病理学影响
影响因子：
3.1
作者：
I. Ohishi;Y. Odagiri
通讯作者：
Y. Odagiri
急性霍乱患者空肠前列腺素 E2 浓度增加。
影响因子：
24.5
作者：
P. Speelman;G. Rabbani;K. Bukhave;J. Rask
通讯作者：
J. Rask
艰难梭菌产生的两种毒素的比较
影响因子：
3.1
作者：
N. Taylor;G. Thorne;J. Bartlett
通讯作者：
J. Bartlett
影响因子：
15.9
作者：
HECHT, G;POTHOULAKIS, C;MADARA, JL
通讯作者：
MADARA, JL