Development of a novel photoreactive calmodulin derivative: cross-linking of purified adenylate cyclase from bovine brain.
Development of a novel photoreactive calmodulin derivative: cross-linking of purified adenylate cyclase from bovine brain.
复制标题
新型光反应性钙调蛋白衍生物的开发：来自牛脑的纯化腺苷酸环化酶的交联。
DOI：
10.1021/bi00440a045
复制
发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Gnegy,ME
中科院分区：
文献类型：
--
作者：
Harrison,JK;Lawton,RG;Gnegy,ME
Departments of Pharmacology and Chemistry, The University of Michigan, Ann Arbor, Michigan 48109 Received October 3, 1988; Revised Manuscript Received March 14, 1989 abstract: A novel photoreactive calmodulin (CaM) derivative was developed and used to label the purified CaM-sensitive adenylate cyclase from bovine cortex. 125I-CaM was conjugated with the heterobifunctional cross-linking agent p-nitrophenyl 3-diazopyruvate (DAPpNP). Spectral data indicated that diazopyruvoyl (DAP) groups were incorporated into the CaM molecule. Iodo-CaM-DAPs behaved like native CaM with respect to (1) Ca2+-dependent enhanced mobility on sodium dodecyl sulfate-polyacrylamide gels and (2) Ca2+-dependent stimulation of adenylatecyclase activity. 125I-CaM-DAP photochemically cross-linked to CaM-binding proteins in a manner that was both Ca2+ dependent and CaM specific. Photolysis of forskolin-agarose-purified adenylatecyclase from bovine cortex with 125I-CaM-DAP produced a single cross-linked product which migrates on sodium dodecyl sulfate-polyacrylamide gels with an apparent molecular weight of approximately 140000.
登录
查看更多内容
脑中两种不同的腺苷酸环化酶通过单克隆抗体进行区分。
DOI：
--
发表时间：
1988
期刊：
European Journal of Biochemistry
影响因子：
--
作者：
S. Mollner;T. Pfeuffer
通讯作者：
T. Pfeuffer
DOI：
10.1016/0006-291x(82)90712-4
发表时间：
1982-01-01
影响因子：
3.1
作者：
GOPALAKRISHNA, R;ANDERSON, WB
通讯作者：
ANDERSON, WB
牛脑钙调蛋白敏感腺苷酸环化酶的催化亚基与 125I 标记的麦芽凝集素和 125I 标记的钙调蛋白之间的直接相互作用。
影响因子：
2.9
作者：
Minocherhomjee,M;Selfe,S;Flowers,NJ;Storm,DR
通讯作者：
Storm,DR
牛或大鼠脑的环状3:5-核苷酸磷酸二酯酶的蛋白质激活剂也激活其腺苷酸环化酶。
DOI：
10.1016/0006-291x(75)90747-0
发表时间：
1975
影响因子：
3.1
作者：
W. Y. Cheung;W. Y. Cheung;L. S. Bradham;Thomas J. Lynch;Ying Ming Lin;E.Ann Tallant
通讯作者：
E.Ann Tallant
叠氮钙调蛋白与红细胞膜 (Ca2 Mg2 )-ATP 酶的光化学交联。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hinds,TR;Andreasen,TJ
通讯作者：
Andreasen,TJ