Single-cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cord.
Single-cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cord.
复制标题
受损小鼠脊髓中细胞异质性和相互作用的单细胞分析。
DOI：
10.1084/jem.20210040
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发表时间：
2021-08-02
期刊：
影响因子：
--
通讯作者：
Lee JK
中科院分区：
文献类型：
--
作者：
Milich LM;Choi JS;Ryan C;Cerqueira SR;Benavides S;Yahn SL;Tsoulfas P;Lee JK
这项研究使用单细胞 RNA 测序来研究已知构成小鼠急性损伤脊髓的所有细胞类型的转录异质性。作者研究了细胞亚型异质性以及骨髓细胞、血管细胞和大胶质细胞之间潜在的信号相互作用。脊髓损伤后发生的伤口愈合过程对于维持组织稳态和限制组织损伤至关重要，但最终会导致不利于再生和修复的疤痕样环境。更好地理解这种二分法对于开发针对适当病理学的有效疗法至关重要，但主要挑战是巨大的细胞异质性导致极其复杂的细胞相互作用。在这项研究中，我们使用单细胞 RNA 测序来评估几乎所有构成小鼠脊髓损伤部位的细胞类型。除了发现新的亚群之外，我们还使用受体-配体对的表达值来识别预计调节血管生成、神经胶质增生和纤维化过程中特定细胞相互作用的信号通路。我们的数据集是一个宝贵的资源，它不仅为脊髓损伤而且为中枢神经系统的其他创伤性疾病的病理学提供了新颖的机制见解。
This study uses single-cell RNA sequencing to investigate the transcriptional heterogeneity of all cell types known to comprise the acutely injured spinal cord in mice. The authors investigate both cell subtype heterogeneity and potential signaling interactions between myeloid, vascular, and macroglia cells. The wound healing process that occurs after spinal cord injury is critical for maintaining tissue homeostasis and limiting tissue damage, but eventually results in a scar-like environment that is not conducive to regeneration and repair. A better understanding of this dichotomy is critical to developing effective therapeutics that target the appropriate pathobiology, but a major challenge has been the large cellular heterogeneity that results in immensely complex cellular interactions. In this study, we used single-cell RNA sequencing to assess virtually all cell types that comprise the mouse spinal cord injury site. In addition to discovering novel subpopulations, we used expression values of receptor–ligand pairs to identify signaling pathways that are predicted to regulate specific cellular interactions during angiogenesis, gliosis, and fibrosis. Our dataset is a valuable resource that provides novel mechanistic insight into the pathobiology of not only spinal cord injury but also other traumatic disorders of the CNS.
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