Identification of a conserved neutralizing epitope in Seneca Valley virus VP2 protein: new insight for epitope vaccine designment.
Identification of a conserved neutralizing epitope in Seneca Valley virus VP2 protein: new insight for epitope vaccine designment.
复制标题
塞内卡谷病毒VP2蛋白中一个保守中和表位的鉴定：表位疫苗设计的新见解
DOI：
10.1186/s12985-022-01791-5
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发表时间：
2022-04-11
期刊：
影响因子：
4.8
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Wen W;Chen X;Lv Q;Chen H;Qian P;Li X
塞尼卡谷病毒（SVV）是一种引起猪水泡病的小核糖核酸病毒。该病的临床特征与口蹄疫病毒、猪水泡病病毒、水泡性口炎病毒等常见病毒性疾病相似，可引起猪的鼻子或蹄部出现水泡。因此，开发SVV感染的检测工具是至关重要和紧迫的。产生中和抗体以检测中和表位。用超纯化的SVV-LNSY 01 -2017免疫小鼠，制备了5株SVV中和性单克隆抗体（mAb），命名为2C 8、3E 4、4C 3、6D 7和7 C11。所有五种单克隆抗体对SVV均表现出高中和滴度。通过使用GST融合肽的肽扫描进一步鉴定这些mAb靶向的表位。肽153 QELNEE 158被定义为最小线性中和表位。抗体显示对VP 2单突变体E157 A没有反应性。此外，抗体对重组病毒（SVV-E157 A）没有显示中和活性。这5株单克隆抗体及其所鉴定的抗原表位为进一步研究VP 2的结构和功能以及建立SVV不同毒株的诊断方法奠定了基础。此外，VP 2蛋白的单克隆抗体识别的表位可能为新型SVV疫苗和溶瘤病毒的开发提供见解。
Seneca Valley virus (SVV) is a picornavirus that causes vesicular disease in swine. Clinical characteristics of the disease are similar to common viral diseases such as foot-and-mouth disease virus, porcine vesicular disease virus, and vesicular stomatitis virus, which can cause vesicles in the nose or hoof of pigs. Therefore, developing tools for detecting SVV infection is critical and urgent. The neutralizing antibodies were produced to detect the neutralizing epitope. Five SVV neutralizing monoclonal antibodies (mAb), named 2C8, 3E4, 4C3, 6D7, and 7C11, were generated by immunizing mouses with ultra-purified SVV-LNSY01-2017. All five monoclonal antibodies exhibited high neutralizing titers to SVV. The epitopes targeted by these mAbs were further identified by peptide scanning using GST fusion peptides. The peptide 153QELNEE158 is defined as the smallest linear neutralizing epitope. The antibodies showed no reactivity to VP2 single mutants E157A. Furthermore, the antibodies showed no neutralizing activity with the recombinant virus (SVV-E157A). The five monoclonal antibodies and identified epitopes may contribute to further research on the structure and function of VP2 and the development of diagnostic methods for detecting different SVV strains. Additionally, the epitope recognized by monoclonal antibodies against VP2 protein may provide insights for novel SVV vaccines and oncolytic viruses development.
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DOI：
10.1016/j.str.2008.07.013
发表时间：
2008-10-08
期刊：
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影响因子：
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作者：
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影响因子：
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影响因子：
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影响因子：
4.8
作者：
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影响因子：
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作者：
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