Early repeated maternal separation induces alterations of hippocampus reelin expression in rats
Early repeated maternal separation induces alterations of hippocampus reelin expression in rats
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早期反复母体分离导致大鼠海马 reelin 表达的改变
DOI：
10.1007/s12038-012-9286-3
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发表时间：
2013-03
影响因子：
2.9
通讯作者：
Zhao, Hu
中科院分区：
文献类型：
--
作者：
Zhang, Jianlong;Qin, Lina;Zhao, Hu
本研究探讨了出生后早期反复母亲分离（MS）对发育中大鼠海马reelin表达的长期影响。通过在出生后第2-14天（PND）每天将Wistar大鼠幼仔单独与其母亲分离3小时进行MS。在PND 22、PND 60和PND 90时，使用qRT-PCR和蛋白质印迹法测定海马中的Reelin mRNA和蛋白质水平。MS导致发育期大鼠体重下降，海马中reelin mRNA和蛋白水平在发育期普遍下调，但90日龄雄性大鼠海马中reelin mRNA和蛋白水平上调。这些发现表明，MS对海马reelin mRNA和蛋白质表达水平的长期影响取决于收集应激大鼠大脑的年龄; reelin对正常大脑发育所需的母婴相互作用具有重要意义。结论：出生后早期反复发生的MS可能导致发育期大鼠海马reelin表达随年龄增长而改变。
The long-term effects of repeated maternal separation (MS) during early postnatal life on reelin expression in the hippocampus of developing rats were investigated in the present study. MS was carried out by separating Wistar rat pups singly from their mothers for 3 h a day during postnatal days (PND) 2–14. Reelin mRNA and protein levels in the hippocampus were determined using qRT-PCR and Western blotting, at PND 22, PND 60 and PND 90. MS resulted in the loss of body weight in the developing rats, and reelin mRNA and protein levels in the hippocampus generally were down-regulated over the developing period, but the reelin mRNA and protein levels in the hippocampus of 90-day-old male rats were up-regulated. These findings suggest that the long-term effects of MS on the expression levels of hippocampal reelin mRNA and protein depends on the age at which the stressed rats’ brains were collected; reelin had important implications for the maternal-neonate interaction needed for normal brain development. In conclusion, repeated MS occurring during early postnatal life may cause the alterations of hippocampal reelin expression with the increasing age of developing rats.
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影响因子：
3.2
作者：
A. Schore
通讯作者：
A. Schore
影响因子：
2.7
作者：
Lina Qin;Wen-qiang Tu;Xiumin Sun;Jianlong Zhang;Yuchuang Chen;Hu Zhao
通讯作者：
Lina Qin;Wen-qiang Tu;Xiumin Sun;Jianlong Zhang;Yuchuang Chen;Hu Zhao
影响因子：
4.7
作者：
I. Weaver;R. Grant;M. Meaney
通讯作者：
I. Weaver;R. Grant;M. Meaney
影响因子：
2.5
作者：
Qiu, Shenfeng;Weeber, Edwin J.
通讯作者：
Weeber, Edwin J.
影响因子：
2.9
作者：
Matthews, Keith;Wilkinson, Lawrence S.;Robbins, Trevor W.
通讯作者：
Robbins, Trevor W.