Diatoms and Plants Acyl-CoA:lysophosphatidylcholine Acyltransferases (LPCATs) Exhibit Diverse Substrate Specificity and Biochemical Properties.
Diatoms and Plants Acyl-CoA:lysophosphatidylcholine Acyltransferases (LPCATs) Exhibit Diverse Substrate Specificity and Biochemical Properties.
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硅藻和植物酰基辅酶 A：溶血磷脂酰胆碱酰基转移酶 (LPCAT) 表现出多种底物特异性和生化特性
DOI：
10.3390/ijms22169056
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发表时间：
2021-08-22
影响因子：
5.6
通讯作者：
Banaś A
中科院分区：
文献类型：
--
作者：
Połońska A;Jasieniecka-Gazarkiewicz K;You L;Hao X;Klińska S;Gong Y;Banaś A
三角褐指藻基因组数据库的搜索显示存在6个基因可能编码溶血磷脂酰基转移酶。这些基因之一，Phatr3_J20460，在引入LPCAT基因中被破坏的酵母ale 1突变体后，产生了非常活跃的酰基辅酶A：溶血磷脂酰胆碱（LPCAT）酶。使用在体外试验中应用不同的放射性和非放射性底物和微粒体组分从这样的酵母，我们的特点是生化特性和底物特异性的PtLPCAT 1。我们已经发现，这种酶的底物特异性表明，它可以完全提供磷脂酰胆碱（PC）与所有脂肪酸连接的非常长链的多不饱和脂肪酸（VLC-PUFA）的生物合成途径进一步用于去饱和过程。此外，我们已经表明，PtLPCAT 1与植物LPCAT相比的生物化学性质在某些情况下是相似的（如其活性对pH值的依赖性），适度不同（如对温度变化的反应），或表达完全不同的性质（如对钙和镁离子的反应或对一些具有20 C多不饱和脂肪酸的酰基辅酶A的反应）。此外，所获得的结果表明，克隆的“Phatr3_J20460”基因可用于油料种子植物工程，以有效生产VLC-PUFA，因为它编码的LPCAT可（与植物LPCAT相反）将20：4-CoA（n-3）引入PC以进一步去饱和至20：5（EPA，二十碳五烯酸）。
The search of the Phaeodactylum tricornutum genome database revealed the existence of six genes potentially encoding lysophospholipid acyltransferases. One of these genes, Phatr3_J20460, after introduction to yeast ale1 mutant disrupted in the LPCAT gene, produced a very active acyl-CoA:lysophosphatidylcholine (LPCAT) enzyme. Using in vitro assays applying different radioactive and non-radioactive substrates and microsomal fractions from such yeast, we have characterized the biochemical properties and substrate specificities of this PtLPCAT1. We have found that the substrate specificity of this enzyme indicates that it can completely supply phosphatidylcholine (PC) with all fatty acids connected with a biosynthetic pathway of very long-chain polyunsaturated fatty acids (VLC-PUFAs) used further for the desaturation process. Additionally, we have shown that biochemical properties of the PtLPCAT1 in comparison to plant LPCATs are in some cases similar (such as the dependency of its activity on pH value), differ moderately (such as in response to temperature changes), or express completely different properties (such as in reaction to calcium and magnesium ions or toward some acyl-CoA with 20C polyunsaturated fatty acids). Moreover, the obtained results suggest that cloned “Phatr3_J20460” gene can be useful in oilseeds plant engineering toward efficient production of VLC-PUFA as LPCAT it encodes can (contrary to plant LPCATs) introduce 20:4-CoA (n-3) to PC for further desaturation to 20:5 (EPA, eicosapentaenoic acid).
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影响因子：
5.4
作者：
Dolch LJ;Maréchal E
通讯作者：
Maréchal E
影响因子：
5
作者：
Mus, Florence;Toussaint, Jean-Paul;Carlson, Ross P.
通讯作者：
Carlson, Ross P.
影响因子：
5.4
作者：
Mühlroth A;Li K;Røkke G;Winge P;Olsen Y;Hohmann-Marriott MF;Vadstein O;Bones AM
通讯作者：
Bones AM
影响因子：
4.8
作者：
Pan, Xue;Chen, Guanqun;Weselake, Randall J.
通讯作者：
Weselake, Randall J.
影响因子：
5.6
作者：
Mapelli-Brahm, Ana;Sanchez, Rosario;Venegas-Caleron, Monica
通讯作者：
Venegas-Caleron, Monica