AvaII and BglI restriction maps of bacteriophage Mu.
AvaII and BglI restriction maps of bacteriophage Mu.
复制标题
噬菌体 Mu 的 AvaII 和 BglI 限制图谱。
DOI:
10.1016/s0042-6822(83)80013-0
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发表时间:
1983
期刊:
影响因子:
3.7
通讯作者:
Howe,MM
中科院分区:
文献类型:
--
作者:
Marrs,CF;Howe,MM
TheAvaII andBglI restriction maps of bacteriophage Mu were derived by restrictionanalysis of a series of plasmid clones containing segments of Mu DNA which, in combination, covered the entire Mu genome. The plasmids analyzed included pKN36, pKN54, pKN62, pKN50, pKN35, pKN27, pKN48, pKN82, and pKN56 from the collection of W. Schumann and E. G. Bade, and pCM02, a newly constructed plasmid containing the rightmost internalEcoRI-PstI fragment of Mu DNA.BglI cuts Mu DNA at 23 sites, producing 24 fragments which range in size from 0.05 kb up to the approximately 7-kb fragment derived from the right end.AvaII cuts Mu DNA at 17 sites (including 2 within the G segment), producing fragments which range in size from 0.17 to 8.9 kb. The derived maps were confirmed by results of hybridization of32P-labeled, nick-translated plasmid DNA toAvaII- andBglI-digested Mu DNAs. Evidence for modification of one of theAvaII sites inE. coliwas obtained.
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