Mars Organic Detector III: a versatile instrument for detection of bio-organic signatures on Mars
Mars Organic Detector III: a versatile instrument for detection of bio-organic signatures on Mars
复制标题
火星有机探测器 III：用于探测火星生物有机特征的多功能仪器
DOI：
10.1117/12.520580
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
R. Mathies
中科院分区：
文献类型：
--
作者：
A. Skelley;F. Grunthaner;J. Bada;R. Mathies
微制造芯片实验室分析系统的最新进展提高了原位化学和生化分析仪的可行性和能力。虽然可以探测各种各样的生物有机分子，但我们的初步研究重点是开发一种氨基酸分析仪，并假设外星生命将基于同手性氨基酸聚合物。在之前的工作中，我们开发了一种原型电泳芯片、检测系统和分析方法，用荧光素标记水解氨基酸，然后在γ-环糊精作为手性识别剂存在下，通过毛细管区带电泳（CZE）分离在几分钟内进行分析。在最近的工作中，我们已经证明了使用荧光胺作为标记试剂进行氨基酸组成和手性分析的可行性。荧光胺具有优势，因为它与氨基酸反应更快，荧光背景低，而且这种化学物质将直接与斯克里普斯正在开发的火星有机探测器（MOD-I）概念相结合。一个更先进的分析系统，称为MOD-III，这里介绍了分析两性离子氨基酸，核碱基，糖，有机酸和碱基使用新的捕获基质化学的能力。MOD-III由纳升阀、泵和反应器组成，将提供广泛的有机化学分析，适用于各种原位任务。
Recent advances in the development of microfabricated lab-on-a-chip analysis systems have enhanced the feasibility and capabilities of in situ chemical and biochemical analyzers. While a wide variety of bio-organic molecules can be probed, we have focused our initial studies on the development of an amino acid analyzer with the hypothesis that extraterrestrial life would be based on homochiral amino acid polymers. In previous work, we developed a prototype electrophoresis chip, detection system and analysis method where the hydrolyzed amino acids were labeled with fluorescein and then analyzed in minutes via a capillary zone electrophoresis (CZE) separation in the presence of γ-cyclodextrin as the chiral recognition agent. In more recent work, we have demonstrated the feasibility of performing amino acid composition and chirality analyses using fluorescamine as the labeling reagent. Fluorescamine is advantageous because it reacts more rapidly with amino acids, has a low fluorescence background and because such a chemistry would interface directly with the Mars Organic Detector (MOD-I) concept being developed at Scripps. A more advanced analysis system called MOD-III is introduced here with the ability to analyze zwitterionic amino acids, nucleobases, sugars, and organic acids and bases using novel capture matrix chemistries. MOD-III, which is enabled by the nanoliter valves, pumps and reactors presented here, will provide a wide spectrum of organic chemical analyses and is suitable for a variety of in situ missions.
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影响因子：
7.4
作者：
Lagally, ET;Medintz, I;Mathies, RA
通讯作者：
Mathies, RA
影响因子：
7.4
作者：
Emrich, CA;Tian, HJ;Mathies, RA
通讯作者：
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影响因子：
4.1
作者：
Sykora, D;Svec, F;Fréchet, JMJ
通讯作者：
Fréchet, JMJ
影响因子：
7.4
作者：
Paegel, BM;Yeung, SHI;Mathies, RA
通讯作者：
Mathies, RA