Lower expressions of the human bitter taste receptor TAS2R in smokers: reverse transcriptase-polymerase chain reaction analysis.
Lower expressions of the human bitter taste receptor TAS2R in smokers: reverse transcriptase-polymerase chain reaction analysis.
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DOI：
10.1186/1617-9625-12-12
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发表时间：
2014
影响因子：
3.7
通讯作者：
Suganuma N
中科院分区：
文献类型：
--
作者：
Aoki M;Takao T;Takao K;Koike F;Suganuma N
尽管吸烟者对味觉，尤其是苦味的感知能力存在缺陷，但目前还没有研究调查其生物学相关性。在此背景下，我们比较了吸烟者和非吸烟者舌头中苦味受体基因，味觉2受体（TAS2R）的表达。研究人员从22名吸烟者和22名年龄和性别匹配的无吸烟史的健康志愿者（19名男性和3名女性）的舌头侧面收集了组织样本。采用逆转录-聚合酶链反应检测两组TAS2R的表达，并评估衰老对TAS2R表达的影响。吸烟者TAS2R表达明显低于非吸烟者（t = 6.525, P < 0.05）。0001, 11.36±6.0 vs. 2.09±2.8,mean±SD，不吸烟者vs.吸烟者)。此外，在非吸烟者中，年龄与TAS2R的表达呈正相关(r =。642, p =。001)，但吸烟者没有(r =。124, p = .584)。相关性差异有统计学意义（Z = 1.96, P = 0.0496）。与不吸烟者相比，吸烟者苦味受体基因的表达明显低于不吸烟者，这可能是由于吸烟导致他们无法随着年龄的增长获得这种受体。
Despite the fact that smokers have deficit in detecting taste, particularly bitter taste, no study has investigated its biological correlate. In this context, we compared the expression of the bitter taste receptor gene, taste 2 receptor (TAS2R) in the tongues of smokers and non-smokers. Tissue samples were collected from the lateral portion of the tongues of 22 smokers and 22 age- and gender-matched healthy volunteers (19 males and three females) with no history of smoking. Reverse transcriptase-polymerase chain reaction was used to examine the expression of TAS2R in the two groups, and the effect of aging on TAS2R expression was also assessed. TAS2R expression was significantly lower among smokers than non-smokers (t = 6.525, P < .0001, 11.36 ± 6.0 vs. 2.09 ± 2.8, mean ± SD, non-smokers vs. smokers). Further, a positive correlation between age and expression of TAS2R was observed in non-smokers (r = .642, P = .001), but not smokers (r = .124, P = .584). This correlation difference was significant (Z = 1.96, P = .0496). Smokers showed a significantly lower expression of the bitter taste receptor gene than non-smokers, which is potentially caused by their inability to acquire such receptors with age because of cigarette smoking, in contrast to non-smokers.
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影响因子：
168.9
作者：
Fowkes, F. Gerald R.;Rudan, Diana;Criqui, Michael H.
通讯作者：
Criqui, Michael H.
DOI：
10.1080/01639360802261920
发表时间：
2008-01-01
期刊：
Journal of Nutrition for the Elderly
影响因子：
--
作者：
Navarro-Allende, Alejandra;Khataan, Nora;El-Sohemy, Ahmed
通讯作者：
El-Sohemy, Ahmed
影响因子：
3.7
作者：
Cao S;Yin X;Wang Y;Zhou H;Song F;Lu Z
通讯作者：
Lu Z
影响因子：
2.6
作者：
Sekine, Hiroki;Takao, Kyoichi;Ikeda, Minoru
通讯作者：
Ikeda, Minoru
影响因子：
3.3
作者：
Tomassini, S.;Cuoghi, V.;Bigiani, A.
通讯作者：
Bigiani, A.