Absence of glyphosate-induced effects on ovarian folliculogenesis and steroidogenesis.
Absence of glyphosate-induced effects on ovarian folliculogenesis and steroidogenesis.
复制标题
DOI：
10.1016/j.reprotox.2020.06.011
复制
发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
Keating AF
中科院分区：
文献类型：
--
作者：
Ganesan S;McGuire BC;Keating AF
甘氨酸（GLY）是一种除草活性成分，体外和体内研究表明，GLY改变卵巢功能。为了确定慢性GLY暴露模型是否影响体内类固醇生成或卵泡生成，出生后第42天的C57 BL 6雌性小鼠每周5天从移液管尖端经口递送载体对照（盐水）或GLY（2 mg/Kg），持续5周或10周。在发情周期的发情前期对小鼠实施安乐死。GLY暴露不影响体重增加、器官重量或健康卵泡数量。此外，GLY暴露不影响卵巢mRNA编码kit配体（Kitlg）、KIT原癌基因受体酪氨酸激酶（c-Kit）、胰岛素受体（Insr）、胰岛素受体底物（Irs 1或Irs 2）和蛋白胸腺瘤病毒原癌基因1（AKT）或磷酸化AKT的丰度。GLY对卵巢星星、3β-羟基类固醇脱氢酶（Hsd 3b 1）、Cyp 11 a1和Cyp 19 a的mRNA或蛋白质丰度也没有影响。循环17β-雌二醇和孕酮浓度不受GLY暴露的影响。总之，慢性GLY暴露5或10周不影响本文检查的卵巢终点。
Glyphosate (GLY) is an herbicidal active ingredient and both in vitro and in vivo studies suggest that GLY alters ovarian function. To determine if a chronic GLY exposure model affected steroidogenesis or folliculogenesis in vivo, postnatal day 42 C57BL6 female mice were orally delivered vehicle control (saline) or GLY (2 mg/Kg) from a pipette tip five days per week for either five or ten weeks. Mice were euthanized at the pro-estrus stage of the estrous cycle. GLY exposure did not impact body weight gain, organ weights, or healthy follicle numbers. In addition, GLY exposure did not affect abundance of ovarian mRNA encoding kit ligand (Kitlg), KIT proto-oncogene receptor tyrosine kinase (c-Kit), insulin receptor (Insr), insulin receptor substrate (Irs1 or Irs2) and protein thymoma viral proto-oncogene 1 (AKT) or phosphorylated AKT. Ovarian mRNA or protein abundance of Star, 3β-hydroxysteroid dehydrogenase (Hsd3b1), Cyp11a1 or Cyp19a were also not altered by GLY. Circulating 17β-estradiol and progesterone concentration were unaffected by GLY exposure. In conclusion, chronic GLY exposure for five or ten weeks did not affect the ovarian endpoints examined herein.
登录
查看更多内容
影响因子：
3.8
作者：
Keating AF;J Mark C;Sen N;Sipes IG;Hoyer PB
通讯作者：
Hoyer PB
影响因子：
8.8
作者：
Perego, Maria Chiara;Caloni, Francesca;Spicer, Leon J.
通讯作者：
Spicer, Leon J.
影响因子：
4.5
作者：
Gasnier, Celine;Dumont, Coralie;Seralini, Gilles-Eric
通讯作者：
Seralini, Gilles-Eric
影响因子：
3.6
作者：
Brown C;LaRocca J;Pietruska J;Ota M;Anderson L;Smith SD;Weston P;Rasoulpour T;Hixon ML
通讯作者：
Hixon ML
影响因子：
6
作者：
Parvez, S.;Gerona, R. R.;Winchester, P. D.
通讯作者：
Winchester, P. D.