First complete genome sequence of European turkey coronavirus suggests complex recombination history related with US turkey and guinea fowl coronaviruses.
First complete genome sequence of European turkey coronavirus suggests complex recombination history related with US turkey and guinea fowl coronaviruses.
复制标题
DOI：
10.1099/jgv.0.000338
复制
发表时间：
2016-01
期刊：
影响因子：
--
通讯作者：
Eterradossi N
中科院分区：
文献类型：
--
作者：
Brown PA;Touzain F;Briand FX;Gouilh AM;Courtillon C;Allée C;Lemaitre E;De Boisséson C;Blanchard Y;Eterradossi N
欧洲火鸡冠状病毒（TCoV）分离株的基因组全长为27739 nt。总体而言，ORF的顺序、数量和大小与其他γ冠状病毒一致。三个点的重组进行了预测，一个接近年底的1a，第二个在1b的上游的S和第三个在3b。对这三点定义的四个区域的系统发育分析支持了之前的观点，即欧洲和美国的病毒确实有不同的进化途径。在除一个地区外的所有地区，欧洲TCoV和欧洲珍珠鸡冠状病毒之间的关系都非常密切，并且两者都与欧洲传染性支气管炎病毒（IBV）意大利2005密切相关。这些序列区域均未将欧洲和美国的TCoV分组。包含S基因的序列区域在将所有火鸡和珍珠鸡冠状病毒分组在一起，将它们与IBV分开方面是独特的。有趣的是，法国珍珠鸡病毒与北美病毒的关系更为密切。这些数据表明，欧洲火鸡和珍珠鸡冠状病毒共享一个共同的遗传骨架（最有可能是IBV意大利2005的祖先），并表明这在两个单独的事件中与不同但相关的未知禽冠状病毒重组，获得了它们的S-3a基因。数据还显示，北美病毒与欧洲火鸡和珍珠鸡病毒没有共同的骨架;然而，它们与珍珠鸡病毒有相似的S-3a基因。
A full-length genome sequence of 27 739 nt was determined for the only known European turkey coronavirus (TCoV) isolate. In general, the order, number and size of ORFs were consistent with other gammacoronaviruses. Three points of recombination were predicted, one towards the end of 1a, a second in 1b just upstream of S and a third in 3b. Phylogenetic analysis of the four regions defined by these three points supported the previous notion that European and American viruses do indeed have different evolutionary pathways. Very close relationships were revealed between the European TCoV and the European guinea fowl coronavirus in all regions except one, and both were shown to be closely related to the European infectious bronchitis virus (IBV) Italy 2005. None of these regions of sequence grouped European and American TCoVs. The region of sequence containing the S gene was unique in grouping all turkey and guinea fowl coronaviruses together, separating them from IBVs. Interestingly the French guinea fowl virus was more closely related to the North American viruses. These data demonstrate that European turkey and guinea fowl coronaviruses share a common genetic backbone (most likely an ancestor of IBV Italy 2005) and suggest that this recombined in two separate events with different, yet related, unknown avian coronaviruses, acquiring their S-3a genes. The data also showed that the North American viruses do not share a common backbone with European turkey and guinea fowl viruses; however, they do share similar S-3a genes with guinea fowl virus.
登录
查看更多内容
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.7
作者：
Jackwood MW;Boynton TO;Hilt DA;McKinley ET;Kissinger JC;Paterson AH;Robertson J;Lemke C;McCall AW;Williams SM;Jackwood JW;Byrd LA
通讯作者：
Byrd LA
影响因子：
6.5
作者：
Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
2.7
作者：
Hughes AL
通讯作者：
Hughes AL
影响因子：
2.8
作者：
ANDRAL, B;TOQUIN, D
通讯作者：
TOQUIN, D