Degradation Products of Complex Arabinoxylans by Bacteroides intestinalis Enhance the Host Immune Response.
Degradation Products of Complex Arabinoxylans by Bacteroides intestinalis Enhance the Host Immune Response.
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DOI：
10.3390/microorganisms9061126
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发表时间：
2021-05-22
期刊：
影响因子：
4.5
通讯作者：
Cann I
中科院分区：
文献类型：
--
作者：
Yasuma T;Toda M;Abdel-Hamid AM;D'Alessandro-Gabazza C;Kobayashi T;Nishihama K;D'Alessandro VF;Pereira GV;Mackie RI;Gabazza EC;Cann I
拟杆菌属人类结肠微生物组可降解膳食纤维中的复杂阿拉伯木聚糖并释放阿魏酸。多项研究已经证明阿魏酸的有益作用。在这里，我们假设在复杂的阿拉伯木聚糖存在下培养的阿魏酸或富含阿魏酸的肠道拟杆菌培养物上清液可以增强免疫反应。与对照组相比，阿魏酸和在不溶性阿拉伯木聚糖存在下培养的细菌的培养物上清液显着降低了肿瘤坏死因子-α的表达，并增加了活化树突状细胞的白细胞介素-10和转化生长因子β1的表达。在用阿魏酸或用不溶性阿拉伯木聚糖培养的细菌的培养上清液处理的小鼠中，肠系膜淋巴结中的粒细胞数量、脾单核细胞/粒细胞数量以及白细胞介素2和白细胞介素12血浆水平显着增加。阿魏酸或与不溶性阿拉伯木聚糖一起培养的细菌的培养物上清液增加了肠上皮细胞系中白介素-12、干扰素-α和干扰素-β的表达。这项研究表明，与不溶性阿拉伯木聚糖一起培养的结肠细菌肠拟杆菌的阿魏酸或富含阿魏酸的培养物上清液在炎症条件下发挥树突状细胞的抗炎活性，并增强小鼠生理条件下的Th1型免疫反应。
Bacteroides spp. of the human colonic microbiome degrade complex arabinoxylans from dietary fiber and release ferulic acid. Several studies have demonstrated the beneficial effects of ferulic acid. Here, we hypothesized that ferulic acid or the ferulic acid-rich culture supernatant of Bacteroides intestinalis, cultured in the presence of complex arabinoxylans, enhances the immune response. Ferulic acid and the culture supernatant of bacteria cultured in the presence of insoluble arabinoxylans significantly decreased the expression of tumor necrosis factor-α and increased the expression of interleukin-10 and transforming growth factor β1 from activated dendritic cells compared to controls. The number of granulocytes in mesenteric lymph nodes, the number of spleen monocytes/granulocytes, and interleukin-2 and interleukin-12 plasma levels were significantly increased in mice treated with ferulic acid or the culture supernatant of bacteria cultured with insoluble arabinoxylans. Ferulic acid or the culture supernatant of bacteria cultured with insoluble arabinoxylans increased the expression of interleukin-12, interferon-α, and interferon-β in intestinal epithelial cell lines. This study shows that ferulic acid or the ferulic acid-rich culture supernatant of the colonic bacterium Bacteroides intestinalis, cultured with insoluble arabinoxylans, exerts anti-inflammatory activity in dendritic cells under inflammatory conditions and enhances the Th1-type immune response under physiological conditions in mice.
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影响因子：
64.8
作者：
Glenwright AJ;Pothula KR;Bhamidimarri SP;Chorev DS;Baslé A;Firbank SJ;Zheng H;Robinson CV;Winterhalter M;Kleinekathöfer U;Bolam DN;van den Berg B
通讯作者：
van den Berg B
聚糖代谢如何塑造人类肠道微生物群。
DOI：
10.1038/nrmicro2746
发表时间：
2012-04-11
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4
作者：
Couteau, D;McCartney, AL;Faulds, CB
通讯作者：
Faulds, CB
影响因子：
9.8
作者：
Martens EC;Lowe EC;Chiang H;Pudlo NA;Wu M;McNulty NP;Abbott DW;Henrissat B;Gilbert HJ;Bolam DN;Gordon JI
通讯作者：
Gordon JI
影响因子：
4.8
作者：
Holck, Jesper;Fredslund, Folmer;Wilkens, Casper
通讯作者：
Wilkens, Casper