Effects of different stocking densities on tracheal barrier function and its metabolic changes in finishing broilers.
Effects of different stocking densities on tracheal barrier function and its metabolic changes in finishing broilers.
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DOI：
10.1016/j.psj.2020.09.026
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发表时间：
2020-12
期刊：
影响因子：
4.4
通讯作者：
Zhan X
中科院分区：
文献类型：
--
作者：
Wang Y;Wang D;Wang J;Li K;Heng C;Jiang L;Cai C;Zhan X
本研究旨在探讨不同饲养密度对肉鸡气管屏障功能及血浆代谢的影响。将1,440只22日龄岭南黄羽肉鸡随机分为5个不同饲养密度组（8 m−2、10 m−2、12 m−2、14 m−2和16 m−2）。每个样本均由3个重复样本组成。16 m−2治疗组的白细胞介素（IL）-1β和IL-10浓度显著高于8 m−2和10 m−2治疗组（P < 0.05）。而IL-4在5个处理组间无显著性差异（P > 0.05）。16 m−2组（高放养密度，HSD）的鸟类气管粘膜明显厚于10 m−2组（对照，CSD）的鸟类。相对于CSD，HSD的claudin 1表达水平较低，muc 2和caspase 3表达水平较高。与CSD相比，HSD组有10种代谢产物表达显著上调（P < 0.05），7种代谢产物表达显著下调（P < 0.05）。这些推定的诊断生物标志物中的大多数涉及物质生物合成和能量代谢。代谢途径分析显示，最相关和最关键的生物标志物是戊糖和葡萄糖醛酸相互转化和戊糖磷酸途径。上述途径的激活可能部分抵消了高放养密度引起的应激的不利影响。这项工作有助于提高我们对高饲养密度对气管屏障的有害影响的理解，并确定了2个代谢途径，可能与高饲养密度诱导的代谢紊乱的肉鸡。
In the present study, we evaluated the effects of various stocking densities on the tracheal barrier and plasma metabolic profiles of finishing broilers. We randomly assigned 1,440 Lingnan Yellow feathered broilers (age 22 d) to 5 different stocking density groups (8 m−2, 10 m−2, 12 m−2, 14 m−2, and 16 m−2). Each of these consisted of 3 replicates. The interleukin (IL)-1β and IL-10 concentrations were substantially higher in the 16 m−2 treatment group than they were in the 8 m−2 and 10 m−2 treatment groups (P < 0.05). Nevertheless, IL-4 did not significantly differ among the 5 treatments (P > 0.05). The tracheal mucosae of the birds in the 16 m−2 group (high stocking density, HSD) were considerably thicker than those for the birds in the 10 m−2 group (control, CSD). Relative to CSD, the claudin1 expression level was lower, and the muc2 and caspase3 expression levels were higher for HSD. Compared with CSD, 10 metabolites were significantly upregulated (P < 0.05), and 7 were significantly downregulated (P < 0.05) in HSD. Most of these putative diagnostic biomarkers were implicated in matter biosynthesis and energy metabolism. A metabolic pathway analysis revealed that the most relevant and critical biomarkers were pentose and glucuronate interconversions and the pentose phosphate pathway. Activation of the aforementioned pathways may partially counteract the adverse effects of the stress induced by high stocking density. This work helped improve our understanding of the harmful effects of high stocking density on the tracheal barrier and identified 2 metabolic pathways that might be associated with high stocking density–induced metabolic disorders in broilers.
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影响因子：
4.6
作者：
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通讯作者：
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DOI：
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发表时间：
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期刊：
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作者：
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影响因子：
64.8
作者：
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通讯作者：
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DOI：
10.5713/ajas.18.0939
发表时间：
2019-12-01
期刊：
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
SUNDE, ML