Multiple consecutive recapture of rigid nanoparticles using a solid-state nanopore sensor.
Multiple consecutive recapture of rigid nanoparticles using a solid-state nanopore sensor.
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DOI：
10.1002/elps.201700329
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发表时间：
2018-03
期刊：
影响因子：
2.9
通讯作者：
Kim M
中科院分区：
文献类型：
--
作者：
Lee JS;Peng B;Sabuncu AC;Nam S;Ahn C;Kim MJ;Kim M
固体纳米孔传感器已被用于测量纳米颗粒的大小，通过应用电阻脉冲传感技术。以前，可以利用单个易位的数据来调查种群池的大小分布，但由于缺乏重复数据，分布的准确性受到限制。在这项研究中，我们利用单颗粒重捕技术对聚苯乙烯纳米球进行了表征，该技术比单采样技术提供了更好的尺寸分布统计估计。使用定制脚本控制的自动化系统多次捕获纳米颗粒，反复获取两种不同尺寸纳米颗粒（直径为80 nm和125 nm）的脉冲和易位次数。通过求解漂移扩散方程，得到了捕获系统构型参数的良好估计。实验结果表明，由于纳米颗粒被多次捕获，测量精度和准确度得到了提高。讨论了固态纳米孔中再捕获和电容效应的互易性。我们的研究结果表明，固态纳米孔和自动再捕获系统也可以应用于软纳米颗粒，如脂质体、外泌体或病毒，以单颗粒分辨率分析它们的机械特性。
Solid-state nanopore sensors have been used to measure the size of a nanoparticle by applying a resistive pulse sensing technique. Previously, the size distribution of the population pool could be investigated utilizing data from a single translocation, however, the accuracy of the distribution is limited due to the lack of repeated data. In this study, we characterized polystyrene nanobeads utilizing single particle recapture techniques, which provide a better statistical estimate of the size distribution than that of single sampling techniques. The pulses and translocation times of two different sized nanobeads (80 nm and 125 nm in diameter) were acquired repeatedly as nanobeads were recaptured multiple times using an automated system controlled by custom-built scripts. The drift-diffusion equation was solved to find good estimates for the configuration parameters of the recapture system. The results of the experiment indicated enhancement of measurement precision and accuracy as nanobeads were recaptured multiple times. Reciprocity of the recapture and capacitive effects in solid state nanopores are discussed. Our findings suggest that solid-state nanopores and an automated recapture system can also be applied to soft nanoparticles, such as liposomes, exosomes, or viruses, to analyze their mechanical properties in single-particle resolution.
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