Colitis induces calcitonin gene-related peptide expression and Akt activation in rat primary afferent pathways.
Colitis induces calcitonin gene-related peptide expression and Akt activation in rat primary afferent pathways.
复制标题
DOI：
10.1016/j.expneurol.2009.04.026
复制
发表时间：
2009-09
影响因子：
5.3
通讯作者：
Grider, John R.
中科院分区：
文献类型：
--
作者：
Qiao, Li-Ya;Grider, John R.
Previous study has shown that colitis-induced increases in calcitonin gene-related peptide (CGRP) immunoreactivity in bladder afferent neurons result in sensory cross-sensitization. To further determine the effects of colitis on CGRP expression in neurons other than bladder afferents, we examined and compared the levels of CGRP mRNA and immunoreactivity in the lumbosacral dorsal root ganglia (DRG) and spinal cord before and during colitis in rats. We also examined the changes in CGRP immunoreactivity in colonic afferent neurons during colitis. Results showed increases in CGRP mRNA levels in L1 (2.5-fold, p<0.05) and S1 DRG (1.9-2.4-fold, p<0.05). However, there were no changes in CGRP mRNA levels in L1 and S1 spinal cord during colitis. CGRP protein was significantly increased in L1 (2.5-fold increase, p<0.05) but decreased in S1 (50% decrease, p<0.05) colonic afferent neurons, which may reflect CGRP release from these neurons during colitis. In L1 spinal cord, colitis caused increases in the number of CGRP nerve fibers in the deep lamina region extending to the gray commissure where the number of phospho-Akt neurons was also increased. In S1 spinal cord, colitis caused the increases in the intensity of CGRP fibers in the regions of dorsolateral tract, and caused the increases in the level of phospho-Akt in the superficial dorsal horn of the spinal cord. In spinal cord slice culture, exogenous CGRP increased the phosphorylation level of Akt but not the phosphorylation level of extracellular-signal regulated kinase ERK1/2 even though our previously studies showed that colitis increased the phosphorylation level of ERK1/2 in L1 and S1 spinal cord. These results suggest that CGRP is synthesized in the DRG and may transport to the spinal cord where initiates signal transduction during colitis.
登录
查看更多内容
DOI：
10.1152/ajpgi.00464.2006
发表时间：
2007-07-01
影响因子：
4.5
作者：
Clifton, Matthew S.;Hoy, Julia J.;Bhargava, Aditi
通讯作者：
Bhargava, Aditi
影响因子：
--
作者：
EVANGELISTA, S;TRAMONTANA, M
通讯作者：
TRAMONTANA, M
影响因子：
24.5
作者：
Gebhart, GF;Bielefeldt, K;Ozaki, N
通讯作者：
Ozaki, N
影响因子：
29.4
作者：
Brierley, SM;Jones, RCW;Blackshaw, LA
通讯作者：
Blackshaw, LA
影响因子：
--
作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M