Plant U13 orthologues and orphan snoRNAs identified by RNomics of RNA from Arabidopsis nucleoli.
Plant U13 orthologues and orphan snoRNAs identified by RNomics of RNA from Arabidopsis nucleoli.
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DOI：
10.1093/nar/gkp1241
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发表时间：
2010-05
影响因子：
14.9
通讯作者：
Brown JW
中科院分区：
文献类型：
--
作者：
Kim SH;Spensley M;Choi SK;Calixto CP;Pendle AF;Koroleva O;Shaw PJ;Brown JW
小核仁RNAs(SnoRNAs)和小Cajal体特异性RNAs(ScaRNAs)是非编码RNA，其在真核生物中的主要功能分别是指导核糖体和剪接体小核RNA的核苷酸修饰。利用从拟南芥细胞培养物中分离的核仁的RNA，从有封顶和未封顶的小RNA的cDNA文库中获得了拟南芥snoRNAs和scaRNAs的全长序列。我们已经鉴定了31个新的snoRNA基因(9个盒C/D和22个盒H/ACA)和15个先前描述的snoRNA的新变体。三个具有不同基因组织和结构的相关封闭型snoRNAs被鉴定为动物U13snoRNAs的同源基因。此外，其中8个新基因与rRNAs或SnRNAs没有互补性，因此可能是孤儿snoRNAs，可能反映了这些RNAs更广泛的功能。通过原位杂交证实了一些snoRNAs的核仁定位和两个可能的scaRNAs的核体定位。大多数新的snoRNA基因被发现在新的基因簇中或作为先前描述的簇的一部分。这些结果将拟南芥snoRNAs库扩展到188个snoRNA基因和294个基因变体。
Small nucleolar RNAs (snoRNAs) and small Cajal body-specific RNAs (scaRNAs) are non-coding RNAs whose main function in eukaryotes is to guide the modification of nucleotides in ribosomal and spliceosomal small nuclear RNAs, respectively. Full-length sequences of Arabidopsis snoRNAs and scaRNAs have been obtained from cDNA libraries of capped and uncapped small RNAs using RNA from isolated nucleoli from Arabidopsis cell cultures. We have identified 31 novel snoRNA genes (9 box C/D and 22 box H/ACA) and 15 new variants of previously described snoRNAs. Three related capped snoRNAs with a distinct gene organization and structure were identified as orthologues of animal U13snoRNAs. In addition, eight of the novel genes had no complementarity to rRNAs or snRNAs and are therefore putative orphan snoRNAs potentially reflecting wider functions for these RNAs. The nucleolar localization of a number of the snoRNAs and the localization to nuclear bodies of two putative scaRNAs was confirmed by in situ hybridization. The majority of the novel snoRNA genes were found in new gene clusters or as part of previously described clusters. These results expand the repertoire of Arabidopsis snoRNAs to 188 snoRNA genes with 294 gene variants.
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影响因子：
64.5
作者：
KISS, T;MARSHALLSAY, C;FILIPOWICZ, W
通讯作者：
FILIPOWICZ, W
影响因子：
3.5
作者：
Bazeley, Peter S.;Shepelev, Valery;Fedorov, Alexei
通讯作者：
Fedorov, Alexei
影响因子：
11.6
作者：
Drea, S;Leader, DJ;Doonan, JH
通讯作者：
Doonan, JH
影响因子：
3.3
作者：
Chen, Chun-Long;Chen, Chong-Jian;Qu, Liang-Hu
通讯作者：
Qu, Liang-Hu
影响因子：
13.8
作者：
BACHELLERIE, JP;MICHOT, B;FOURNIER, MJ
通讯作者：
FOURNIER, MJ