Myocardial Extracellular Volume Fraction Measured by Cardiac Magnetic Resonance Imaging Negatively Correlates With Cardiomyocyte Breadth in a Healthy Porcine Model.
Myocardial Extracellular Volume Fraction Measured by Cardiac Magnetic Resonance Imaging Negatively Correlates With Cardiomyocyte Breadth in a Healthy Porcine Model.
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通过心脏磁共振成像测量的心肌细胞外体积分数与健康猪模型中的心肌细胞宽度呈负相关
DOI：
10.3389/fcvm.2022.791963
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发表时间：
2022
影响因子：
3.6
通讯作者：
Ju S
中科院分区：
文献类型：
--
作者：
Zhang SJ;Chang D;Jin JY;Wang YL;Wang L;Wang YC;Wang Z;Ju S
心脏磁共振成像（MRI）测量的细胞外容积分数（ECV）被广泛用于评价心肌纤维化。然而，由于在健康个体中的有限的组织学验证，尚不清楚心肌细胞的大小是否可能在ECV的生理变化中发挥潜在的作用。本研究的目的是通过使用健康猪模型来检查心肌细胞大小与心肌ECV之间的关联。16头健康家猪麻醉后机械控制呼吸进行心脏MRI检查。静脉注射造影剂的剂量为0.2-0.25 mmol/kg。使用已建立的MRI程序计算室间隔ECV，该程序基于心脏和个体血液红细胞压积的造影前后T1值。苏木精-伊红染色测定心肌细胞横截面宽度（CmyB），以反映心肌细胞的大小。成功采集了14头猪的数据。CmyB从心脏MRI上感兴趣区域对应的心肌组织中获得。ECV的平均值± SD为0.253 ± 0.043，CmyB的平均值± SD为10.02 ± 0.84 μm。ECV与CmyB呈负相关（r =-0.729，p = 0.003）。在健康猪中，通过心脏MRI检测的心肌ECV与CmyB呈负相关，表明在生理条件下心肌细胞的大小与ECV潜在相关。
The extracellular volume fraction (ECV) derived from cardiac magnetic resonance imaging (MRI) is extensively used to evaluate myocardial fibrosis. However, due to the limited histological verification in healthy individuals, it remains unclear whether the size of cardiomyocytes may play a potential role in the physiological changes of ECV. The aim of this study was to examine the association between cardiomyocyte size and myocardial ECV by using a healthy porcine model. Sixteen domestic healthy pigs were anesthetized and underwent cardiac MRI with mechanical controlled breathing. Intravenous contrast medium was introduced at a dose of 0.2–0.25 mmol/kg. The interventricular septum ECV was calculated using an established MRI procedure, which was based on the pre- and post-contrast T1 values of the heart and individual blood hematocrit. The cardiomyocyte breadth (CmyB) in cross section was measured by hematoxylin and eosin staining to reflect the cardiomyocyte size. Data were successfully acquired from 14 pigs. The CmyB was obtained from the myocardial tissues corresponding to the region of interest on cardiac MRI. The mean ± SD of the ECV was 0.253 ± 0.043, and the mean ± SD of the CmyB was 10.02 ± 0.84 μm. The ECV exhibited a negative correlation with the CmyB (r = −0.729, p = 0.003). The myocardial ECV detected by cardiac MRI is negatively correlated with the CmyB in healthy pigs, demonstrating that the size of cardiomyocytes is potentially associated with the ECV under physiological conditions.
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DOI：
10.1186/s12968-016-0308-4
发表时间：
2016-11-30
期刊：
Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance
影响因子：
--
作者：
Haaf P;Garg P;Messroghli DR;Broadbent DA;Greenwood JP;Plein S
通讯作者：
Plein S
影响因子：
6.2
作者：
Florian, Anca;Ludwig, Anna;Yilmaz, Ali
通讯作者：
Yilmaz, Ali
DOI：
10.1152/ajpheart.00429.2008
发表时间：
2008-09-01
影响因子：
4.8
作者：
Jerosch-Herold, Michael;Sheridan, David C.;Hershberger, Ray E.
通讯作者：
Hershberger, Ray E.
DOI：
10.1186/s12968-018-0445-z
发表时间：
2018-04-23
期刊：
Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance
影响因子：
--
作者：
Cui Y;Cao Y;Song J;Dong N;Kong X;Wang J;Yuan Y;Zhu X;Yan X;Greiser A;Shi H;Han P
通讯作者：
Han P
影响因子：
24
作者：
Martinez-Naharro, Ana;Treibel, Thomas A.;Fontana, Marianna
通讯作者：
Fontana, Marianna