Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm.
Label-free quantitative proteomic analysis of serum exosomes in mice with thoracic aortic aneurysm.
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DOI：
10.1186/s12953-023-00220-x
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发表时间：
2023-10-24
期刊：
影响因子：
2
通讯作者：
Jiang, Yong
中科院分区：
文献类型：
--
作者：
Xu, Jia;Liu, Jiacheng;Qu, Yibai;Jiang, Linhui;Liang, Rongxin;Li, Bohai;Li, Lei;Jiang, Yong
胸主动脉瘤是一种发病率和死亡率都很高的心血管疾病。然而，TAA的原因和机制尚未完全了解。来自患有TAA的小鼠的血清外泌体用于探索与这种疾病相关的标志物。C57 BL/6小鼠分为3组，分别给予普通饮用水、普通饮用水+生理盐水渗透泵和饮用水+ β-氨基丙腈（BAPN）（1 g/kg/d）+血管紧张素II（Ang II）（1 μg/kg/min）渗透泵。对胸主动脉组织进行苏木精和伊红染色。分析了外泌体的基本特征。通过LC-MS/MS鉴定差异表达蛋白（DEPs）。蛋白质组蛋白网络和富集分析用于探索可能的分子机制。本研究阐明了BAPN联合Ang II诱导的TAA小鼠血清exosomes的蛋白表达谱。在这项工作中，共有196种蛋白质的表达在TAA小鼠的血清外泌体中显著失调，其中122种蛋白质显著上调，74种蛋白质显著下调。值得注意的是，基于PPI网络鉴定的触珠蛋白（Hp）和血清淀粉样蛋白p组分（Sap）显著上调，并且与心血管疾病密切相关。有趣的是，京都基因和基因组百科全书（KEGG）途径分析表明，上调和下调的蛋白质参与补体和凝血级联途径。本研究表明，DEPs有可能作为诊断TAA的生物标志物，并提供了一个更全面的了解TAA的病理生理机制。在线版本包含补充材料，可通过10.1186/s12953-023-00220-x获得。
Thoracic aortic aneurysm (TAA) is a cardiovascular disease with high morbidity and mortality. However, the causes and mechanisms of TAA are not fully understood. Serum exosomes from mice with TAA were used to explore the markers associated with this disease. C57BL/6 mice were divided into three groups and given ordinary drinking water, ordinary drinking water plus a saline osmotic pump, or drinking water containing β-aminopropionitrile (BAPN) (1 g/kg/d) plus an angiotensin II (Ang II) (1 μg/kg/min) osmotic pump. Haematoxylin and eosin staining of thoracic aortic tissues was performed. The basic characteristics of exosomes were analysed. Differentially expressed proteins (DEPs) were identified by LC‒MS/MS. Protein‒protein networks and enrichment analysis were used to explore possible molecular mechanisms. The present study elucidated the protein expression profile of serum exosomes in mice with TAA induced by BAPN combined with Ang II. In this work, the expression of a total of 196 proteins was significantly dysregulated in serum exosomes of mice with TAA, with 122 proteins significantly upregulated and 74 proteins markedly downregulated. Notably, Haptoglobin (Hp) and Serum amyloid p-component (Sap) identified based on the PPI network were significantly upregulated and have been strongly linked to cardiovascular disease. Interestingly, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the upregulated and downregulated proteins were involved in the complement and coagulation cascade pathways. This study showed that the identified DEPs have potential as biomarkers for the diagnosis of TAA and provided a more comprehensive understanding of the pathophysiological mechanisms of TAA. The online version contains supplementary material available at 10.1186/s12953-023-00220-x.
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影响因子：
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作者：
Su SA;Xie Y;Fu Z;Wang Y;Wang JA;Xiang M
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影响因子：
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作者：
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DOI：
10.3390/life10120337
发表时间：
2020-12-10
期刊：
Life (Basel, Switzerland)
影响因子：
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作者：
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影响因子：
9.3
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Bouillet, Benjamin