Surviving within the amoebal exocyst: the Mycobacterium avium complex paradigm.
Surviving within the amoebal exocyst: the Mycobacterium avium complex paradigm.
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DOI：
10.1186/1471-2180-10-99
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发表时间：
2010-04-01
期刊：
影响因子：
4.2
通讯作者：
Drancourt M
中科院分区：
文献类型：
--
作者：
Ben Salah I;Drancourt M
大多数环境分枝杆菌先前已被证明能够抵抗自由生活的阿米巴原虫，随后毒性增强，并对抗生素和杀菌剂产生耐药性。禽分枝杆菌复合体（MAC）由栖息在各种生态位的环境生物组成，并表现出显著程度的遗传变异性。我们在此研究了作为环境分枝杆菌模式生物的所有MAC成员的变形虫内位置。将鸟分枝杆菌、胞内分枝杆菌、嵌合分枝杆菌、哥伦比亚分枝杆菌、芳香分枝杆菌、马赛分枝杆菌、铁皮分枝杆菌和布切杜龙分枝杆菌型菌株与自由生活的多食棘阿米巴变形虫株lincoln - ap1共培养。显微镜分析表明分枝杆菌在多食假单胞滋养体的液泡中被吞噬和复制。分枝杆菌进一步被包裹在变形虫囊内，并通过传代培养在囊内存活。电镜观察显示，在被捕获到多食棘球蚴囊3天后，所有MAC成员通常都居住在囊内。结合已发表的数据，这些观察结果表明分枝杆菌在阿米巴耐药细菌中是独特的，居住在囊泡内。
Most of environmental mycobacteria have been previously demonstrated to resist free-living amoeba with subsequent increased virulence and resistance to antibiotics and biocides. The Mycobacterium avium complex (MAC) comprises of environmental organisms that inhabit a wide variety of ecological niches and exhibit a significant degree of genetic variability. We herein studied the intra-ameobal location of all members of the MAC as model organisms for environmental mycobacteria. Type strains for M. avium, Mycobacterium intracellulare, Mycobacterium chimaera, Mycobacterium colombiense, Mycobacterium arosiense, Mycobacterium marseillense, Mycobacterium timonense and Mycobacterium bouchedurhonense were co-cultivated with the free-living amoeba Acanthamoeba polyphaga strain Linc-AP1. Microscopic analyses demonstrated the engulfment and replication of mycobacteria into vacuoles of A. polyphaga trophozoites. Mycobacteria were further entrapped within amoebal cysts, and survived encystment as demonstrated by subculturing. Electron microscopy observations show that, three days after entrapment into A. polyphaga cysts, all MAC members typically resided within the exocyst. Combined with published data, these observations indicate that mycobacteria are unique among amoeba-resistant bacteria, in residing within the exocyst.
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影响因子：
9.4
作者：
Esparcia, Oscar;Navarro, Ferran;Coll, Pere
通讯作者：
Coll, Pere
DOI：
10.1016/j.diagmicrobio.2008.12.002
发表时间：
2009-03-01
影响因子：
2.9
作者：
Bills, Nathan D.;Hinrichs, Steven H.;Iwen, Peter C.
通讯作者：
Iwen, Peter C.
DOI：
10.1073/pnas.0610746104
发表时间：
2007-06-26
影响因子：
11.1
作者：
Danelishvili, Lia;Wu, Martin;Bermudez, Luiz E.
通讯作者：
Bermudez, Luiz E.
DOI：
10.1099/ijs.0.65503-0
发表时间：
2008-10-01
影响因子：
2.8
作者：
Bang, Didi;Herlin, Troels;Thomsen, Vibeke Ostergaard
通讯作者：
Thomsen, Vibeke Ostergaard
DOI：
10.1111/j.1365-2672.1990.tb02904.x
发表时间：
1990-05-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
KILVINGTON, S;PRICE, J
通讯作者：
PRICE, J