Wheel running attenuates microglia proliferation and increases expression of a proneurogenic phenotype in the hippocampus of aged mice.
Wheel running attenuates microglia proliferation and increases expression of a proneurogenic phenotype in the hippocampus of aged mice.
复制标题
DOI：
10.1016/j.bbi.2011.10.006
复制
发表时间：
2012-07
影响因子：
15.1
通讯作者：
Rhodes, Justin S.
中科院分区：
文献类型：
--
作者：
Kohman, Rachel A.;DeYoung, Erin K.;Bhattacharya, Tushar K.;Peterson, Lindsey N.;Rhodes, Justin S.
衰老与低级神经炎症有关，包括引发的小胶质细胞，可能导致神经可塑性和认知功能的缺陷。目前的研究评估了运动是否调节海马齿状回中小胶质细胞的分裂和/或激活状态，因为激活的小胶质细胞可以表达经典的炎症或替代的神经保护表型。我们还评估了海马神经发生，以确定小胶质细胞的变化是否与新的神经元存活有关。成年（3.5个月）和老年（18个月）雄性BALB/c小鼠单独饲养8周，有或没有转轮。在实验的第一天或最后十天期间，小鼠接受溴脱氧尿苷注射以标记分裂细胞。进行免疫荧光以测量小胶质细胞分裂、神经保护指示剂胰岛素样生长因子（IGF-1）的共表达和新神经元存活。新生小胶质细胞的比例在老年小鼠中增加，而在轮跑中减少。跑步增加了表达IGF-1的小胶质细胞的比例，表明运动使小胶质细胞表型向神经保护转变。此外，跑步增强了两个年龄组中新神经元的存活率。研究结果表明，车轮运行可能会减弱小胶质细胞分裂，促进老年小鼠的proneurogenic表型。
Aging is associated with low-grade neuroinflammation including primed microglia that may contribute to deficits in neural plasticity and cognitive function. The current study evaluated whether exercise modulates division and/or activation state of microglia in the dentate gyrus of the hippocampus, as activated microglia can express a classic inflammatory or an alternative neuroprotective phenotype. We also assessed hippocampal neurogenesis to determine whether changes in microglia were associated with new neuron survival. Adult (3.5 months) and aged (18 months) male BALB/c mice were individually housed with or without running wheels for 8 weeks. Mice received bromodeoxyuridine injections during the first or last ten days of the experiment to label dividing cells. Immunofluorescence was conducted to measure microglia division, co-expression of the neuroprotective indicator insulin-like growth factor (IGF-1), and new neuron survival. The proportion of new microglia was increased in aged mice, and decreased from wheel running. Running increased the proportion of microglia expressing IGF-1 suggesting exercise shifts microglia phenotype towards neuroprotection. Additionally, running enhanced survival of new neurons in both age groups. Findings suggest that wheel running may attenuate microglia division and promote a proneurogenic phenotype in aged mice.
登录
查看更多内容
影响因子：
9.3
作者：
Nichol KE;Poon WW;Parachikova AI;Cribbs DH;Glabe CG;Cotman CW
通讯作者：
Cotman CW
影响因子：
2.9
作者：
Mouton, PR;Long, JM;Ingram, DK
通讯作者：
Ingram, DK
影响因子：
3.3
作者：
LAWSON, LJ;PERRY, VH;GORDON, S
通讯作者：
GORDON, S
影响因子：
3.6
作者：
Ehninger, Dan;Wang, Li-Ping;Klempin, Friederike;Romer, Benedikt;Kettenmann, Helmut;Kempermann, Gerd
通讯作者：
Kempermann, Gerd
影响因子：
3.4
作者：
Gemma, Carmelina;Bachstetter, Adam D.;Bickford, Paula C.
通讯作者：
Bickford, Paula C.