Intimal growth on the luminal surface of arteriovenous grafts in rats
Intimal growth on the luminal surface of arteriovenous grafts in rats
复制标题
大鼠动静脉移植物管腔表面内膜生长
DOI：
10.1007/s10157-023-02320-6
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发表时间：
2023
影响因子：
2.3
通讯作者：
Ito Yasuhiko
中科院分区：
文献类型：
--
作者：
Ono Sumihisa;Hatayama Naoyuki;Miyamoto Kanyu;Naito Munekazu;Ishimoto Takuji;Ito Yasuhiko
背景内皮细胞生长于血液透析中使用的动静脉移植物（AVG）的管腔表面。虽然内皮细胞对于预防感染是重要的，但是AVG中内皮细胞的详细生长是未知的。本研究旨在建立一个更简单的动物模型AVGs，并探讨如何内皮细胞形成的luminal surface.MethodsPolyethylene移植物之间的颈部动脉和静脉的Wistar大鼠。移植物在放置后6 h、24 h、3 d或7 d取出。光镜和偏光显微镜下观察血管表面，内皮细胞标记物（LEL、CD 31）、祖细胞标记物CD 34和巨噬细胞标记物艾德-1染色。虽然在6小时或7天时动脉侧（AS）和静脉侧（VS）之间的LEL阳性细胞数量没有差异，但在24小时和3天时VS上的这些细胞显著更多（p< 0.05）。在24小时的分析显示，一些CD 31阳性细胞和少数CD 34阳性cells.ConclusionsThis是第一次研究使用一个简单的大鼠模型AVG的位置。内皮细胞的形成最初更活跃的VS比AS，但这些细胞随后在整个管腔表面的数量增加。未来的临床研究可能会通过确认AS与VS穿刺是否导致不同的感染率而做出临床贡献。
BackgroundEndothelial cells are known to grow on the luminal surface of arteriovenous grafts (AVGs) used in hemodialysis. Although endothelial cells are important for preventing infection, a detailed growth of endothelial cells in AVGs is unknown. This study sought to create a simpler animal model of AVGs and to investigate how endothelial cells form on the luminal surface.MethodsPolyethylene grafts were placed between the cervical artery and vein of Wistar rats. The grafts were removed at 6 h, 24 h, 3 days, or 7 days after placement. The luminal surface was observed under optical and polarizing microscopy and stained with endothelial cell markers (LEL, CD31), the progenitor cell marker CD34, and the macrophage marker ED-1.ResultsMicroscopy demonstrated many diffuse vascular endothelial cells on the luminal surface of AVGs after placement. While there was no difference in the number of LEL-positive cells between the arterial side (AS) and venous side (VS) at 6 h or 7 days, there were significantly more of these cells on the VS at both 24 h and 3 days (p< 0.05). Analysis at 24 h showed some CD31-positive cells and few CD34-positive cells.ConclusionsThis was the first study to use a simple rat model of AVG placement. Endothelial cell formation was initially more active on the VS than on the AS, but these cells subsequently increased in number across the luminal surface. Future clinical studies might contribute clinically by confirming whether AS versus VS puncture results in different infection rates.
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影响因子：
20.3
作者：
Bhattacharya, V;McSweeney, PA;Wu, MHD
通讯作者：
Wu, MHD
影响因子：
13.2
作者：
Minga, TE;Flanagan, KH;Allon, M
通讯作者：
Allon, M
DOI：
10.1073/pnas.97.7.3422
发表时间：
2000-03
影响因子：
11.1
作者：
C. Kalka;H. Masuda;Tomono Takahashi;W. Kalka-Moll;Marcy Silver;M. Kearney;Tong Li;J. Isner
通讯作者：
C. Kalka;H. Masuda;Tomono Takahashi;W. Kalka-Moll;Marcy Silver;M. Kearney;Tong Li;J. Isner
影响因子：
4.3
作者：
Ryan, SV;Calligaro, KD;Dougherty, MJ
通讯作者：
Dougherty, MJ
影响因子：
2.3
作者：
Debbage, PL;Sölder, E;Kreczy, A
通讯作者：
Kreczy, A