Arabidopsis QWRF1 and QWRF2 Redundantly Modulate Cortical Microtubule Arrangement in Floral Organ Growth and Fertility.
Arabidopsis QWRF1 and QWRF2 Redundantly Modulate Cortical Microtubule Arrangement in Floral Organ Growth and Fertility.
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拟南芥 QWRF1 和 QWRF2 冗余地调节花器官生长和生育力中的皮质微管排列。
DOI：
10.3389/fcell.2021.634218
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发表时间：
2021
影响因子：
5.5
通讯作者：
Zhu L
中科院分区：
文献类型：
--
作者：
Ma H;Xu L;Fu Y;Zhu L
花器官发育是被子植物有性生殖的基础。许多关键的花调控因子（其中大部分是转录因子）已被鉴定并显示出调节花分生组织的确定性和花器官的同一性，但对花器官生长的调控知之甚少，这是器官实现适当形态和履行其功能的关键过程。初始细胞增殖后各向异性细胞扩增的空间和时间控制对于器官生长是重要的。众所周知，皮层微管在植物细胞极性生长/扩展中具有重要作用，并且已报道指导萼片和花瓣的生长和形状。在这项研究中，我们确定了两个同源蛋白，QWRF 1和QWRF 2，这是必不可少的花器官生长和植物育性。我们发现，严重畸形的形态和对称性的各种花器官，以及在qwrf 1 qwrf 2双突变体的结实率显着降低，虽然很少花发育缺陷被认为是在qwrf 1或qwrf 2单突变体。QWRF 1和QWRF 2显示相似的表达模式，并且在体外和体内都定位于微管。此外，我们发现改变皮质微管组织和安排在qwrf 1 qwrf 2细胞，在不同的花器官，这最终导致生育力差的异常细胞扩张一致。我们的研究结果表明，QWRF 1和QWRF 2可能是微管相关蛋白与生育和花器官发育的功能冗余，这可能发挥其作用，通过调节皮层微管和各向异性的细胞扩张。
Floral organ development is fundamental to sexual reproduction in angiosperms. Many key floral regulators (most of which are transcription factors) have been identified and shown to modulate floral meristem determinacy and floral organ identity, but not much is known about the regulation of floral organ growth, which is a critical process by which organs to achieve appropriate morphologies and fulfill their functions. Spatial and temporal control of anisotropic cell expansion following initial cell proliferation is important for organ growth. Cortical microtubules are well known to have important roles in plant cell polar growth/expansion and have been reported to guide the growth and shape of sepals and petals. In this study, we identified two homolog proteins, QWRF1 and QWRF2, which are essential for floral organ growth and plant fertility. We found severely deformed morphologies and symmetries of various floral organs as well as a significant reduction in the seed setting rate in the qwrf1qwrf2 double mutant, although few flower development defects were seen in qwrf1 or qwrf2 single mutants. QWRF1 and QWRF2 display similar expression patterns and are both localized to microtubules in vitro and in vivo. Furthermore, we found altered cortical microtubule organization and arrangements in qwrf1qwrf2 cells, consistent with abnormal cell expansion in different floral organs, which eventually led to poor fertility. Our results suggest that QWRF1 and QWRF2 are likely microtubule-associated proteins with functional redundancy in fertility and floral organ development, which probably exert their effects via regulation of cortical microtubules and anisotropic cell expansion.
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影响因子：
4.6
作者：
Dinneny, JR;Yadegari, R;Weigel, D
通讯作者：
Weigel, D
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影响因子：
11.4
作者：
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影响因子：
11.6
作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.6
作者：
Dinneny, JR;Weigel, D;Yanofsky, MF
通讯作者：
Yanofsky, MF