Extensive microbial and functional diversity within the chicken cecal microbiome.
Extensive microbial and functional diversity within the chicken cecal microbiome.
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DOI：
10.1371/journal.pone.0091941
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Pallen MJ
中科院分区：
文献类型：
--
作者：
Sergeant MJ;Constantinidou C;Cogan TA;Bedford MR;Penn CW;Pallen MJ
鸡是全世界食物和蛋白质的主要来源。饲料转化率和鸡的健康依赖于栖息在鸡肠道（包括盲肠）中的大量未开发的复杂微生物群落。我们通过16S rRNA基因序列对20个盲肠样品中的微生物进行了深层微生物群落分析，并对单个盲肠微生物进行了深入的宏基因组学分析。我们回收了699个物种，其中一半以上似乎代表以前未知的物种。我们获得了648，251个环境基因标签（EGTs），其中大部分代表新物种。这些基因组被分成二十多个基因组草案，其中包括空肠弯曲菌和鸡白痢螺杆菌。我们发现许多多糖和寡糖降解酶编码的宏基因组内，其中一些似乎是多糖利用系统的一部分，遗传证据的协调多糖降解与糖的运输和利用。盲肠宏基因组编码几种发酵途径，导致短链脂肪酸的生产，包括一些新的功能。我们发现了十几种在宏基因组中编码的摄取氢化酶，并推测这些酶在该微生物群落中提供了主要的氢汇，并可能解释该微生物组中几个属的高丰度，包括弯曲杆菌，螺杆菌和巨单胞菌。
Chickens are major source of food and protein worldwide. Feed conversion and the health of chickens relies on the largely unexplored complex microbial community that inhabits the chicken gut, including the ceca. We have carried out deep microbial community profiling of the microbiota in twenty cecal samples via 16S rRNA gene sequences and an in-depth metagenomics analysis of a single cecal microbiota. We recovered 699 phylotypes, over half of which appear to represent previously unknown species. We obtained 648,251 environmental gene tags (EGTs), the majority of which represent new species. These were binned into over two-dozen draft genomes, which included Campylobacter jejuni and Helicobacter pullorum. We found numerous polysaccharide- and oligosaccharide-degrading enzymes encoding within the metagenome, some of which appeared to be part of polysaccharide utilization systems with genetic evidence for the co-ordination of polysaccharide degradation with sugar transport and utilization. The cecal metagenome encodes several fermentation pathways leading to the production of short-chain fatty acids, including some with novel features. We found a dozen uptake hydrogenases encoded in the metagenome and speculate that these provide major hydrogen sinks within this microbial community and might explain the high abundance of several genera within this microbiome, including Campylobacter, Helicobacter and Megamonas.
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影响因子：
3.6
作者：
HEGDE, SN;ROLLS, BA;COATES, ME
通讯作者：
COATES, ME
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A
影响因子：
4.4
作者：
Burt, D. W.
通讯作者：
Burt, D. W.
DOI：
10.1111/j.1432-1033.1997.0590a.x
发表时间：
1997-12-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Bott, M;Pfister, K;Dimroth, P
通讯作者：
Dimroth, P
影响因子：
2
作者：
Hübener, K;Vahjen, W;Simon, O
通讯作者：
Simon, O