Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood.
Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood.
复制标题
来自猪外周血的血液生长内皮细胞（BOEC）的表征。
DOI：
10.3791/63285
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发表时间：
2022-01-06
期刊：
影响因子：
--
通讯作者：
Tefft BJ
中科院分区：
文献类型：
--
作者：
Shradhanjali A;Uthamaraj S;Dragomir-Daescu D;Gulati R;Sandhu GS;Tefft BJ
内皮是一种动态的整体结构，在血管生成、止血、炎症、动态平衡等生理功能中发挥着重要作用。内皮细胞在动脉粥样硬化、高血压和糖尿病等病理生理过程中也起着重要作用。内皮细胞构成血管和淋巴管的内层，在结构和功能上表现出异质性。不同的小组评估了来自人外周血的内皮细胞的功能，重点是来源于造血干细胞的内皮祖细胞或成熟的血液长出的内皮细胞(或内皮细胞集落形成细胞)。这些细胞为治疗和疾病建模提供了自体资源。异种细胞可能提供另一种治疗来源，因为它们的可用性和同质性是通过使用在类似条件下饲养的遗传相似的动物实现的。因此，从猪外周血中分离和扩增高增殖的血管内皮细胞的方法已经被提出。这些细胞可用于心血管组织工程、细胞治疗、疾病建模、药物筛选、内皮细胞生物学研究以及体外共培养以研究异种移植的炎症和凝血反应。
The endothelium is a dynamic integrated structure that plays an important role in many physiological functions such as angiogenesis, hemostasis, inflammation, and homeostasis. The endothelium also plays an important role in pathophysiologies such as atherosclerosis, hypertension, and diabetes. Endothelial cells form the inner lining of blood and lymphatic vessels and display heterogeneity in structure and function. Various groups have evaluated the functionality of endothelial cells derived from human peripheral blood with a focus on endothelial progenitor cells derived from hematopoietic stem cells or mature blood outgrowth endothelial cells (or endothelial colony-forming cells). These cells provide an autologous resource for therapeutics and disease modeling. Xenogeneic cells may provide an alternative source of therapeutics due to their availability and homogeneity achieved by using genetically similar animals raised in similar conditions. Hence, a robust protocol for the isolation and expansion of highly proliferative blood outgrowth endothelial cells from porcine peripheral blood has been presented. These cells can be used for numerous applications such as cardiovascular tissue engineering, cell therapy, disease modeling, drug screening, studying endothelial cell biology, and in vitro co-cultures to investigate inflammatory and coagulation responses in xenotransplantation.
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