Screening preoperative peptide biomarkers for predicting postoperative myocardial infarction after coronary artery bypass grafting.
Screening preoperative peptide biomarkers for predicting postoperative myocardial infarction after coronary artery bypass grafting.
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筛选术前肽生物标志物预测冠状动脉搭桥术后心肌梗死
DOI：
10.1371/journal.pone.0100149
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hong C
中科院分区：
文献类型：
--
作者：
Jiang Z;Hu P;Liu J;Wang D;Jin L;Hong C
术后心肌梗死是心脏手术最严重的并发症之一。目前没有术前生物标志物可用于预测心脏手术后的PMI。在本研究中，我们使用噬菌体展示肽库筛选潜在的术前肽生物标志物，用于预测冠状动脉旁路移植术（CABG）后PMI。20例CABG后发生PMI的患者和20例年龄、性别和体重指数匹配的CABG后未发生PMI的患者被招募为发现队列。另外50名在CABG后发生PMI的患者和50名在CABG后没有发生PMI的随机选择的患者被招募作为验证队列，以验证在发现队列中鉴定的潜在肽生物标志物。50名随机选择的健康志愿者也被纳入验证阶段作为健康对照组。在发现/筛选阶段，随机选择的20个噬菌体克隆中有17个与PMI组的纯化血清IgG发生特异性反应，其中11个来自同一噬菌体克隆，其插入了肽序列GVIMVIAVSCVF（命名为PMI-1）。在验证阶段，噬菌体ELISA显示PMI组90%的患者血清IgG与PMI-1呈阳性反应;相比之下，非PMI组和健康对照组分别只有14%和6%的患者与PMI-1呈阳性反应。PMI-1噬菌体克隆对冠状动脉旁路移植术后发生PMI的敏感性、特异性、阳性预测值、阴性预测值和准确性分别为90.0%、86.0%、86.5%、89.5%和88.0%。PMI组PMI-1噬菌体克隆的吸光度值与术后血清肌钙蛋白I峰值水平呈显著相关（r = 0.349，p =0.012）。 总之，我们第一次确定了一个模拟肽（PMI-1），在冠状动脉旁路移植术后PMI的术前预测具有较高的有效性。
Postoperative myocardial infarction (PMI) is one of the most serious complications of cardiac surgeries. No preoperative biomarker is currently available for predicting PMI after cardiac surgeries. In the present study, we used a phage display peptide library to screen potential preoperative peptide biomarkers for predicting PMI after coronary artery bypass grafting (CABG) surgery. Twenty patients who developed PMI after CABG and 20 age-, sex-, and body mass index-matched patients without PMI after CABG were enrolled as a discovery cohort. Another 50 patients who developed PMI after CABG and 50 randomly selected patients without PMI after CABG were enrolled as a validation cohort to validate the potential peptide biomarkers identified in the discovery cohort. Fifty randomly selected healthy volunteers were also enrolled in the validation phase as a healthy control group. In the discovery/screening phase, 17 out of 20 randomly selected phage clones exhibited specific reaction with purified sera IgG from the PMI group, among which 11 came from the same phage clone with inserted peptide sequence GVIMVIAVSCVF (named PMI-1). In the validation phase, phage ELISA showed that serum IgG from 90% of patients in the PMI group had a positive reaction with PMI-1; in contrast, only 14% and 6% of patients in the non-PMI group and the healthy control group had a positive reaction with PMI-1, respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of the PMI-1 phage clone to preoperatively identify patients who would develop PMI after CABG were 90.0%, 86.0%, 86.5, 89.5% and 88.0%, respectively. The absorbance value of the PMI-1 phage clone showed statistically significant correlation with the peak postoperative serum cardiac troponin I level (r = 0.349,p= 0.012) in the PMI group. In conclusion, we for the first time identified a mimic peptide (PMI-1) with high validity in preoperative prediction of PMI after CABG.
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影响因子：
16.1
作者：
Sergeeva, Anna;Kolonin, Mikhail G.;Molldrem, Jeffrey J.;Pasqualini, Renata;Arap, Wadih
通讯作者：
Arap, Wadih
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影响因子：
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Perez, F