Display technologies: application for the discovery of drug and gene delivery agents.
Display technologies: application for the discovery of drug and gene delivery agents.
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DOI：
10.1016/j.addr.2006.09.018
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发表时间：
2006-12-30
影响因子：
16.1
通讯作者：
Arap, Wadih
中科院分区：
文献类型：
--
作者：
Sergeeva, Anna;Kolonin, Mikhail G.;Molldrem, Jeffrey J.;Pasqualini, Renata;Arap, Wadih
关键词：
识别疾病中细胞表面蛋白质组的分子多样性对于靶向治疗的开发至关重要。靶向治疗的进展需要建立有效的方法来高通量鉴定针对临床相关细胞表面标记物的特异性药物。在过去的十年中，已经开发了许多平台策略来筛选多肽库，以寻找在各种细胞表面蛋白质组背景下选择性表达的受体的配体。鉴定可作为疾病诊断、分析、成像和治疗靶标的配体-受体对的简化程序依赖于展示技术，其中可以在称为生物淘选的过程中根据其与编码核酸的物理连接连续选择具有所需结合特征的多肽。这些技术包括病毒/噬菌体展示、细胞展示、核糖体展示、mRNA展示和共价DNA展示(CDT)，其中噬菌体展示是迄今为止使用最多的。本次综述的范围是展示技术的最新进展，特别强调通过肽噬菌体展示对细胞表面蛋白质组进行分子图谱分析。讨论了来自展示库的靶向化合物在靶向药物和基因治疗载体的发现中的潜在应用。
Recognition of molecular diversity of cell surface proteomes in disease is essential for the development of targeted therapies. Progress in targeted therapeutics requires establishing effective approaches for high-throughput identification of agents specific for clinically relevant cell surface markers. Over the past decade, a number of platform strategies have been developed to screen polypeptide libraries for ligands targeting receptors selectively expressed in the context of various cell surface proteomes. Streamlined procedures for identification of ligand-receptor pairs that could serve as targets in disease diagnosis, profiling, imaging and therapy have relied on the display technologies, in which polypeptides with desired binding profiles can be serially selected, in a process called biopanning, based on their physical linkage with the encoding nucleic acid. These technologies include virus/phage display, cell display, ribosomal display, mRNA display and covalent DNA display (CDT), with phage display being by far the most utilized. The scope of this review is the recent advancements in the display technologies with a particular emphasis on molecular mapping of cell surface proteomes with peptide phage display. Prospective applications of targeted compounds derived from display libraries in the discovery of targeted drugs and gene therapy vectors are discussed.
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影响因子：
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影响因子：
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作者：
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通讯作者：
Sage, EH
DOI：
10.1073/pnas.93.5.1820
发表时间：
1996-03-05
影响因子：
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作者：
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影响因子：
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DOI：
10.1073/pnas.241655998
发表时间：
2002-02-05
影响因子：
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作者：
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通讯作者：
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