Screening for Lactobacillus plantarum Strains That Possess Organophosphorus Pesticide-Degrading Activity and Metabolomic Analysis of Phorate Degradation.
Screening for Lactobacillus plantarum Strains That Possess Organophosphorus Pesticide-Degrading Activity and Metabolomic Analysis of Phorate Degradation.
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具有有机磷农药降解活性的植物乳杆菌菌株筛选及甲拌磷降解代谢组学分析
DOI：
10.3389/fmicb.2018.02048
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发表时间：
2018
影响因子：
5.2
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Li C;Ma Y;Mi Z;Huo R;Zhou T;Hai H;Kwok LY;Sun Z;Chen Y;Zhang H
对121株乳杆菌（Lactobacillus（L.）植物菌株六湖发现植物根瘤菌菌株（P9、IMAU 80110、IMAU 40100、IMAU 10585、IMAU 10209和IMAU 80070）具有高的降解三种常用的有机磷农药（即乐果、甲拌磷和氧乐果）的能力;并且选择它们进行更详细的表征。此外，三种OPPs主要在培养上清液中检测到，而在细胞提取物中未检测到，进一步证实了OPPs被降解而不是被细胞吸收。在6株筛选出的菌株中，P9对胃肠液和胆汁的耐受性最强。因此，我们使用超高效液相色谱电子喷雾电离结合飞行时间质谱（UPLC/ESI-Q-TOF/MS）生成的代谢谱的MRS培养基中生长的菌株P9含有和不含甲拌磷。通过正交偏最小二乘判别分析，我们确定了一些潜在的甲拌磷衍生的降解产物。本研究为农药降解中的乳酸菌资源的开发提供了新的途径。研究结果也为L. plantarum P9.
This work performed a large scale assessment for organophosphorus pesticides (OPPs) degradation activity of 121 Lactobacillus (L.) plantarum strains. Six L. plantarum strains (P9, IMAU80110, IMAU40100, IMAU10585, IMAU10209, and IMAU80070) were found to possess high capacity of degrading three commonly used OPPs, namely dimethoate, phorate, and omethoate; and they were selected for more detailed characterization. Moreover, the three OPPs were mainly detected in the culture supernatants but not in the cell extracts, further confirming that the OPPs were degraded rather than absorbed by the cells. Among the six selected strains, P9 was most tolerant to gastrointestinal juices and bile. We thus used ultra-high performance liquid chromatography electron spray ionization coupled with time-of-flight mass spectrometry (UPLC/ESI-Q-TOF/MS) to generate the metabolomic profiles of the strain P9 growing in MRS medium with and without containing phorate. By using orthogonal partial least squares discriminant analysis, we identified some potential phorate-derived degradative products. This work has identified novel lactic acid bacteria resources for application in pesticide degradation. Our results also shed light on the phorate degradation mechanism by L. plantarum P9.
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影响因子：
8.3
作者：
Debost-Legrand, Anne;Warembourg, Charline;Cordier, Sylvaine
通讯作者：
Cordier, Sylvaine
影响因子：
2.4
作者：
Dordevic, T. M.;Siler-Marinkovic, S. S.;Umiljendic, J. S. Gajic
通讯作者：
Umiljendic, J. S. Gajic
影响因子：
3.6
作者：
Buratti, FM;Testai, E
通讯作者：
Testai, E
影响因子：
2.6
作者：
Clair, Emilie;Linn, Laura;Panoff, Jean-Michel
通讯作者：
Panoff, Jean-Michel
影响因子：
5.8
作者：
Jariyal, Monu;Gupta, V. K.;Singh, Balwinder
通讯作者：
Singh, Balwinder