Performance of commercial dengue NS1 ELISA and molecular analysis of NS1 gene of dengue viruses obtained during surveillance in Indonesia.
Performance of commercial dengue NS1 ELISA and molecular analysis of NS1 gene of dengue viruses obtained during surveillance in Indonesia.
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DOI：
10.1186/1471-2334-13-611
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发表时间：
2013-12-29
影响因子：
3.7
通讯作者：
Sasmono RT
中科院分区：
文献类型：
--
作者：
Aryati A;Trimarsanto H;Yohan B;Wardhani P;Fahri S;Sasmono RT
登革热感染的早期诊断对于更好地管理这种疾病至关重要。以检测登革病毒(DENV)非结构蛋白1(NS1)抗原为基础的诊断试验可以在不同的环境中观察到不同的敏感性和特异性。登革热在印度尼西亚流行，临床医生越来越多地使用NS1检测来确诊登革热。这项研究描述了我们在印度尼西亚8个城市进行监测期间，Panbio登革热早期NS1和IgM捕获ELISA法检测登革热的性能，以及DENV NS1基因的遗传多样性及其与NS1检测的关系。在2010-2012年的登革热监测期间，采用NS1和IgM/Ig G双抗体夹心法进行登革热感染筛查和确认。对采集的血清标本(n = 440)进行逆转录聚合酶链式反应和病毒分离，确诊为登革热感染的标本188份。将检测的阳性结果与RT-PCR结果进行相关性分析，以确定检测的敏感性。对48株印度尼西亚病毒分离株的NS1基因进行了序列测定，并对其基因特征进行了研究。以分子数据为金标准，NS1-EL ISA检测印尼血样的敏感性为56.4%，Ig M-EL ISA为73.7%。当NS1和IgM联合检测时，敏感度提高到89.4%。NS1的敏感性因城市/地理来源和DENV血清型而异，其中DENV-4的敏感性最低(19.0%)。NS1在原发感染中的敏感性(67.6%)高于继发感染(48.2%)。NS1试验对非登革热标本的特异性为100%。对48株分离株的NS1基因序列分析表明，NS1基因存在多态性，对NS1的敏感性无明显影响。我们观察到，在RT-PCR阳性的登革热样本中，NS1-EL ISA检测登革热的敏感性相对较低。当NS1数据与IgM结合时，其检出率显著提高。在我们的研究中，NS1抗原检测的低敏感性与NS1基因多样性无关。相反，NS1抗原检测的效果受到患者感染状况和样本地理来源的影响。
Early diagnosis of dengue infection is crucial for better management of the disease. Diagnostic tests based on the detection of dengue virus (DENV) Non Structural Protein 1 (NS1) antigen are commercially available with different sensitivities and specificities observed in various settings. Dengue is endemic in Indonesia and clinicians are increasingly using the NS1 detection for dengue confirmation. This study described the performance of Panbio Dengue Early NS1 and IgM Capture ELISA assays for dengue detection during our surveillance in eight cities in Indonesia as well as the genetic diversity of DENV NS1 genes and its relationship with the NS1 detection. The NS1 and IgM/IgG ELISA assays were used for screening and confirmation of dengue infection during surveillance in 2010–2012. Collected serum samples (n = 440) were subjected to RT-PCR and virus isolation, in which 188 samples were confirmed for dengue infection. The positivity of the ELISA assays were correlated with the RT-PCR results to obtain the sensitivity of the assays. The NS1 genes of 48 Indonesian virus isolates were sequenced and their genetic characteristics were studied. Using molecular data as gold standard, the sensitivity of NS1 ELISA assay for samples from Indonesia was 56.4% while IgM ELISA was 73.7%. When both NS1 and IgM results were combined, the sensitivity increased to 89.4%. The NS1 sensitivity varied when correlated with city/geographical origins and DENV serotype, in which the lowest sensitivity was observed for DENV-4 (19.0%). NS1 sensitivity was higher in primary (67.6%) compared to secondary infection (48.2%). The specificity of NS1 assay for non-dengue samples were 100%. The NS1 gene sequence analysis of 48 isolates revealed the presence of polymorphisms of the NS1 genes which apparently did not influence the NS1 sensitivity. We observed a relatively low sensitivity of NS1 ELISA for dengue detection on RT-PCR-positive dengue samples. The detection rate increased significantly when NS1 data was combined with IgM. In our study, the low sensitivity of NS1 antigen detection did not relate to NS1 genetic diversity. Rather, the performance of the NS1 antigen test was affected by the infection status of patients and geographical origin of samples.
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登革热快速测试测定的诊断灵敏度通过使用组合的抗原和抗体测试方法可显着增强。
影响因子：
3.8
作者：
Fry SR;Meyer M;Semple MG;Simmons CP;Sekaran SD;Huang JX;McElnea C;Huang CY;Valks A;Young PR;Cooper MA
通讯作者：
Cooper MA
影响因子：
64.8
作者：
IGARASHI, A
通讯作者：
IGARASHI, A
影响因子：
3.1
作者：
Klungthong, C.;Putnak, R.;Zhang, C.
通讯作者：
Zhang, C.
影响因子：
6.4
作者：
Libraty, DH;Young, PR;Rothman, AL
通讯作者：
Rothman, AL
影响因子：
10.7
作者：
Huelsenbeck, JP;Larget, B;Alfaro, ME
通讯作者：
Alfaro, ME