Fluorescent light exposure incites acute and prolonged immune responses in zebrafish (Danio rerio) skin.
Fluorescent light exposure incites acute and prolonged immune responses in zebrafish (Danio rerio) skin.
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DOI：
10.1016/j.cbpc.2017.09.009
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Walter RB
中科院分区：
文献类型：
--
作者：
Gonzalez TJ;Lu Y;Boswell M;Boswell W;Medrano G;Walter S;Ellis S;Savage M;Varga ZM;Lawrence C;Sanders G;Walter RB
人造光产生的发射光谱与太阳光谱大不相同。人造光已被证明会影响脊椎动物的各种行为和生理过程。然而，关于人工光暴露的分子遗传效应的数据很少。先前的研究表明，一种常用的荧光光源（FL; 4，100 K或“冷白色”）可以影响雄性剑尾鱼皮肤中与维持昼夜节律、细胞周期进程、染色体分离和DNA修复/重组相关的信号通路。这些观察结果提出了有关FL诱导的基因表达反应动力学的问题，以及哪些生物学功能在光暴露后的不同时间受到调节。为了解决这些问题，我们将斑马鱼暴露于4，100 K FL，并利用RNA-Seq评估FL暴露后不同时间（1至12小时）皮肤中的基因表达变化。我们发现4，100 K FL激发了一个强大的早期（1-2小时）转录反应，随后是一个更持久的晚期反应（即，4-12小时）。早期转录反应涉及与细胞迁移/浸润和细胞增殖相关的基因，作为免疫功能和炎症总体增加的一部分。延长的晚期转录反应发生在预测维持和延续炎症反应以及抑制脂质、异生物质和褪黑激素代谢的基因组内。
Artificial light produces an emission spectrum that is considerably different than the solar spectrum. Artificial light has been shown to affect various behavior and physiological processes in vertebrates. However, there exists a paucity of data regarding the molecular genetic effects of artificial light exposure. Previous studies showed that one of the commonly used fluorescent light source (FL; 4,100 K or “cool white”) can affect signaling pathways related to maintenance of circadian rhythm, cell cycle progression, chromosome segregation, and DNA repair/recombination in the skin of male Xiphophorus maculatus. These observations raise questions concerning the kinetics of the FL induced gene expression response, and which biological functions become modulated at various times after light exposure. To address these questions, we exposed zebrafish to 4,100 K FL and utilized RNA-Seq to assess gene expression changes in skin at various times (1 to 12 hrs) after FL exposure. We found 4,100 K FL incites a robust early (1–2 hrs) transcriptional response, followed by a more protracted late response (i.e., 4–12 hrs). The early transcriptional response involves genes associated with cell migration/infiltration and cell proliferation as part of an overall increase in immune function and inflammation. The protracted late transcriptional response occurs within gene sets predicted to maintain and perpetuate the inflammatory response, as well as suppression of lipid, xenobiotic, and melatonin metabolism.
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DOI：
10.1038/jid.2009.65
发表时间：
2009-09
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1111/j.1600-0749.2005.00267.x
发表时间：
2005-10-01
期刊：
PIGMENT CELL RESEARCH
影响因子：
--
作者：
Ban, E;Kasai, A;Oshima, N
通讯作者：
Oshima, N
影响因子：
16.8
作者：
Judson, Olivia P.
通讯作者：
Judson, Olivia P.
DOI：
10.1016/j.cbpc.2014.02.004
发表时间：
2014-06-01
影响因子：
3.9
作者：
Contreras, Adam J.;Boswell, Mikki;Walter, Ronald B.
通讯作者：
Walter, Ronald B.
影响因子：
10.3
作者：
McGrath, J. A.;Robinson, M. K.;Binder, R. L.
通讯作者：
Binder, R. L.