miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis.
miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis.
复制标题
拟南芥叶片发育过程中，miR396靶向的AtGRF转录因子是协调细胞分裂和分化所必需的
DOI：
10.1093/jxb/erq307
复制
发表时间：
2011-01
影响因子：
6.9
通讯作者：
Cui X
中科院分区：
文献类型：
--
作者：
Wang L;Gu X;Xu D;Wang W;Wang H;Zeng M;Chang Z;Huang H;Cui X
在植物中，细胞增殖和极化细胞分化沿着近轴-远轴轴在原基是叶形态建成的关键，而这两个过程之间的时空关系仍然在很大程度上未被探索。在这里，据报道，microRNA 396（miR 396）-靶向拟南芥生长调节因子（AtGRF）所需的叶近轴-远轴极性在拟南芥。AtGRF基因的表达减少转基因miR 396过表达的叶极性突变体不对称learning 1（as 1）和as 2导致植物增强叶近轴-远轴缺陷，作为减少细胞增殖的结果。此外，转基因miR 396过表达显著降低细胞分裂活性和细胞周期相关基因的表达，但导致具有较高倍性水平的叶细胞的百分比增加，表明miR 396通过控制进入有丝分裂细胞周期来负调节细胞增殖。miR 396主要在细胞分裂停滞的叶细胞中表达，与其在细胞周期调控中的作用一致。这些结果共同表明，由miR 396靶向AtGRF介导的细胞分裂活性对于拟南芥叶形态发生期间沿着近轴-远轴的极化细胞分化是重要的。
In plants, cell proliferation and polarized cell differentiation along the adaxial–abaxial axis in the primordium is critical for leaf morphogenesis, while the temporal–spatial relationships between these two processes remain largely unexplored. Here, it is reported that microRNA396 (miR396)-targeted Arabidopsis growth-regulating factors (AtGRFs) are required for leaf adaxial–abaxial polarity in Arabidopsis. Reduction of the expression of AtGRF genes by transgenic miR396 overexpression in leaf polarity mutants asymmetric leaves1 (as1) and as2 resulted in plants with enhanced leaf adaxial–abaxial defects, as a consequence of reduced cell proliferation. Moreover, transgenic miR396 overexpression markedly decreased the cell division activity and the expression of cell cycle-related genes, but resulted in an increased percentage of leaf cells with a higher ploidy level, indicating that miR396 negatively regulates cell proliferation by controlling entry into the mitotic cell cycle. miR396 is mainly expressed in the leaf cells arrested for cell division, coinciding with its roles in cell cycle regulation. These results together suggest that cell division activity mediated by miR396-targeted AtGRFs is important for polarized cell differentiation along the adaxial–abaxial axis during leaf morphogenesis in Arabidopsis.
登录
查看更多内容
影响因子：
7.2
作者：
Colón-Carmona, A;You, R;Doerner, P
通讯作者：
Doerner, P
影响因子：
64.5
作者：
HULSKAMP, M;MISERA, S;JURGENS, G
通讯作者：
JURGENS, G
影响因子：
11.6
作者：
Huang, Weihua;Pi, Limin;Huang, Hai
通讯作者：
Huang, Hai
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
2.7
作者：
Donnelly, PM;Bonetta, D;Dengler, NG
通讯作者：
Dengler, NG