Differential expression of signaling pathways in odontogenic differentiation of ectomesenchymal cells isolated from the first branchial arch
Differential expression of signaling pathways in odontogenic differentiation of ectomesenchymal cells isolated from the first branchial arch
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第一鳃弓外间充质细胞牙源分化中信号通路的差异表达
DOI：
10.1007/s11010-011-0714-8
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发表时间：
2011-01
影响因子：
4.3
通讯作者：
Liu, Lei
中科院分区：
文献类型：
--
作者：
Yu, Yongchun;Li, Mingheng;Sun, Jie;Yang, Miaomiao;Long, Jie;Tian, Weidong;Tang, Wei;Li, Tangxin;Liu, Lei
本研究采用真实的时间RT-PCR芯片技术筛选胚胎第10天（E10）小鼠第一鳃弓外胚间充质细胞牙向分化过程中信号通路的差异表达。通过细胞形态学观察、免疫细胞化学和RT-PCR鉴定细胞来源。然后用真实的实时RT-PCR芯片检测两个不同发育阶段动物细胞中信号通路的差异表达。这些基因分别代表Wnt、Hedgehog、TGF-β、NF-κB和LDL信号通路的主要成员。本研究确定这些信号通路对第一鳃弓外胚间充质细胞的牙向分化是重要的。
The aim of this study was to screen for differential expression of signaling pathways in odontogenic differentiation of ectomesenchymal cells isolated from the first branchial arch of embryonic day 10 (E10) mice by real time RT-PCR microarray. Observations of cellular morphology, immunocytochemistry, and RT-PCR were used to identify the cell source. A real time RT-PCR microarray was then used to detect the differential expression of signaling pathways in cells dissected from animals at two different developmental stages. These assays identified 25 up-regulated genes and 16 down-regulated genes involved in odontogenic differentiation of the ectomesenchymal cells of the first branchial arch. They represented the main members of Wnt, Hedgehog, TGF-β, NF-κB, and LDL signaling pathways. This study determined that these signaling pathways are important for odontogenic differentiation of ectomesenchymal cells of the first branchial arch.
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影响因子：
4.6
作者：
J. Jernvall;T. Åberg;P. Kettunen;S. Keränen;Irma Thesleff
通讯作者：
J. Jernvall;T. Åberg;P. Kettunen;S. Keränen;Irma Thesleff
影响因子：
3
作者：
J. Courtney;J. Blackburn;P. Sharpe
通讯作者：
J. Courtney;J. Blackburn;P. Sharpe
影响因子：
3
作者：
Courtney, JM;Blackburn, J;Sharpe, PT
通讯作者：
Sharpe, PT
影响因子：
8.4
作者：
B. Swift;E. McColl;J. Steele;N. Jepson;I. Steen
通讯作者：
B. Swift;E. McColl;J. Steele;N. Jepson;I. Steen
DOI：
10.1089/omi.2009.0018
发表时间：
2009-08
期刊：
Omics : a journal of integrative biology
影响因子：
--
作者：
Jingang Xiao;Hai Dong;Yao Wu;W. Tian;Lei Liu
通讯作者：
Jingang Xiao;Hai Dong;Yao Wu;W. Tian;Lei Liu