Circular RNA GLIS2 promotes colorectal cancer cell motility via activation of the NF-κB pathway.
Circular RNA GLIS2 promotes colorectal cancer cell motility via activation of the NF-κB pathway.
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环状 RNA GLIS2 通过激活 NF-κB 通路促进结直肠癌细胞运动。
DOI：
10.1038/s41419-020-02989-7
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发表时间：
2020-09-23
影响因子：
9
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Chen J;Yang X;Liu R;Wen C;Wang H;Huang L;Li W;Zhu Z;Zhu Y;Liu H
环状RNA（CircRNA）是一种新发现的非编码RNA家族的生物分子。大量证据表明，circRNA参与了各种癌症的进展。然而，circRNA在结直肠癌（CRC）中的特定功能仍然难以捉摸。在这项研究中，我们研究了三对大肠癌组织和癌旁正常组织中差异表达的circRNA。我们发现circGLIS2在CRC组织和细胞系中表达更高。功能获得和丧失试验表明circGLIS2参与细胞迁移的调节。此外，在CRC细胞中过表达circGLIS2激活NF-κ B通路并诱导促炎趋化因子产生，其通过募集白细胞诱发肿瘤相关炎症。反过来，当癌细胞暴露于circGLIS2过表达癌细胞的上清液时，它们被赋予了迁移和趋化因子产生的能力。此外，拯救试验证实，circGLIS2激活NF-κ B信号传导并通过海绵状吸收miR-671促进细胞迁移。总之，我们的研究表明，circGLIS2作为一个潜在的癌基因，通过miR-671海绵机制维持CRC细胞中NF-κ B信号通路的异常激活状态。本研究为靶向circGLIS2进行结直肠癌干预提供了科学依据。
Circular RNAs (circRNAs) are a newly discovered type of biological molecule that belongs to the noncoding RNA family. Abundant evidence has shown that circRNAs are involved in the progression of various cancers. However, the particular functions of circRNAs in colorectal cancer (CRC) remain elusive. In this study, we investigated the differentially expressed circRNAs in three pairs of cancer tissue and adjacent normal tissue of CRC. We revealed that circGLIS2 expression was higher in CRC tissue and cell lines. Gain-and-loss function assays showed that circGLIS2 was involved in the regulation of cell migration. Moreover, overexpressing circGLIS2 in CRC cells activated the NF-κB pathway and induced pro-inflammatory chemokine production, which evoked tumor-associated inflammation through recruiting leukocytes. In turn, when the cancer cells were exposed to the supernatant of circGLIS2 overexpressed cancer cells, they were endowed with the ability of migration and chemokines production. Furthermore, the rescue assay confirmed that circGLIS2 activated NF-κB signaling and promoted cell migration by sponging miR-671. Overall, our study reveals that circGLIS2, acting as a potential oncogene, maintains the abnormal activation state of the NF-κB signaling pathway via the miR-671 sponge mechanism in CRC cells. This study provides a scientific basis for targeting circGLIS2 in colorectal cancer interventions.
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影响因子：
64.8
作者：
Finisguerra V;Di Conza G;Di Matteo M;Serneels J;Costa S;Thompson AA;Wauters E;Walmsley S;Prenen H;Granot Z;Casazza A;Mazzone M
通讯作者：
Mazzone M
影响因子：
4
作者：
Chi, Yongbing;Luo, Qiancheng;Zhang, Denghai
通讯作者：
Zhang, Denghai
上调的环状 RNA circ-102004 可促进前列腺癌细胞增殖。
DOI：
10.1016/j.ijbiomac.2018.09.076
发表时间：
2019-02-01
影响因子：
8.2
作者：
Jie Si-Tu;Cai, Yi;Li, Zhipeng
通讯作者：
Li, Zhipeng
影响因子：
4.1
作者：
Dudekulay, Dawood B.;Panda, Amaresh C.;Gorospe, Myriam
通讯作者：
Gorospe, Myriam
影响因子：
4.8
作者：
COCQUERELLE, C;MASCREZ, B;BAILLEUL, B
通讯作者：
BAILLEUL, B