Decidualized pseudopregnant rat uterus shows marked reduction in Ang II and Ang-(1-7) levels.
Decidualized pseudopregnant rat uterus shows marked reduction in Ang II and Ang-(1-7) levels.
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DOI：
10.1016/j.placenta.2011.10.016
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发表时间：
2012-01
期刊：
影响因子：
3.8
通讯作者：
Neves, L. A. A.
中科院分区：
文献类型：
--
作者：
Brosnihan, K. B.;Bharadwaj, M. S.;Yamaleyeva, L. M.;Neves, L. A. A.
先前的研究表明，血管紧张素（Ang）II和Ang-（1-7）的浓度降低植入部位在第7天的Sprague-Dawley大鼠相比，紧邻它的网站，没有附着的胚胎，清楚地显示了重要性的胚泡在调节肾素-血管紧张素系统（RAS）。本研究的目的是评估RAS在假孕大鼠（一种不存在孕体的模型）蜕膜化子宫中的调节。切除卵巢的成年雌性大鼠对类固醇治疗的蜕膜细胞反应敏感;通过右侧角的油注射诱导蜕膜化;左侧角作为对照。在蜕膜化和非蜕膜化子宫中检测子宫中Ang I、Ang II和Ang-（1-7）的含量。与非蜕膜化角相比，蜕膜化角中Ang-（1-7）和Ang II以及ACE和ACE 2 mRNA均显著降低。Ang Ⅱ、Ang-（1-7）、ACE和ACE 2的免疫细胞化学特征表明，Ang-（1-7）、Ang Ⅱ和ACE 2通过蜕膜化作用到达抗子宫系膜极。蜕膜化过程中子宫角Ang Ⅱ和Ang-（1-7）含量较非蜕膜化子宫角明显降低。Ang II和Ang-（1-7）与ACE 2在蜕膜角的反系膜极中的不同免疫细胞化学表达，而不是ACE，可能有利于Ang-（1-7）在反系膜极中的形成和作用，该区域在触发蜕膜化过程中起作用。
Previous studies showed that angiotensin (Ang) II and Ang-(1-7) concentrations were reduced in the implantation site at day 7 of pregnancy in Sprague-Dawley rats as compared to the site immediately adjacent to it, which does not have the embryo attached, clearly showing the importance of the blastocyst in the regulation of renin-angiotensin system (RAS). The objective of this study was to evaluate the regulation of the RAS in the decidualized uterus in the pseudopregnant rat, a model without the presence of a conceptus. Ovariectomized, adult female rats were sensitized for the decidual cell reaction with steroid treatments; decidualization was induced by oil-injection of the right horn; the left horn served as a control. The uterine content of Ang I, Ang II, and Ang-(1-7) was examined in the decidualized and non-decidualized uteri. Both Ang-(1-7) and Ang II and ACE and ACE2 mRNA were significantly reduced in the decidualized horn as compared to the non-decidualized horn. Immunocytochemical characterization of Ang II, Ang-(1-7), ACE and ACE2 demonstrated that Ang-(1-7), Ang II, and ACE2 polarize to the anti-mesometrial pole with decidualization. The decidualization process elicits marked reduction in uterine Ang II and Ang-(1-7) content as compared to the non-decidualized horn. The differential immunocytochemical expression of Ang II and Ang-(1-7) with ACE2, but not ACE in the anti-mesometrial pole of the decidualized horn may favor the formation and action of Ang-(1-7) in the anti-mesometrial pole, an area which plays a role in triggering the decidualization process.
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