Intra-blood-brain-barrier synthesis of HTLV-III-specific IgG in patients with neurologic symptoms associated with AIDS or AIDS-related complex.
Intra-blood-brain-barrier synthesis of HTLV-III-specific IgG in patients with neurologic symptoms associated with AIDS or AIDS-related complex.
复制标题
具有与 AIDS 或 AIDS 相关复合体相关的神经系统症状的患者中 HTLV-III 特异性 IgG 的血脑屏障内合成。
DOI：
10.1056/nejm198512123132402
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
R. Gallo
中科院分区：
文献类型：
--
作者：
L. Resnick;Fulvia diMarzo;J. Schüpbach;W. Tourtellotte;D. Ho;F. Müller;P. Shapshak;Markus W. Vogt;J. Groopman;P. Markham;R. Gallo
血脑屏障内病毒特异性抗体的产生是血脑屏障内感染的良好证据。获得性免疫缺陷综合征（AIDS）患者神经系统异常的发生率增加，即，不明原因的弥漫性脑病，临床表现为慢性进行性痴呆。为了确定人类T细胞嗜淋巴细胞病毒III型（HTLV-III），艾滋病的病原体，在神经功能障碍的发病机制中的作用，我们比较了脑脊液和血清与艾滋病相关的神经症状和艾滋病相关的复杂的存在针对HTLV-III的抗体。针对HTLV-III抗原的抗体通过四种免疫学试验检测：固定细胞免疫荧光试验、酶联免疫吸附试验、病毒裂解物免疫印迹和细胞裂解物免疫沉淀。所有患者的血清反应均为阳性，23例患者中有22例（96%）的脑脊液中有HTLV-III特异性抗体。在脑脊液中检测到独特的寡克隆IgG条带，并且血脑屏障内的IgG合成速率升高。在9例患者中的8例中，酶联免疫吸附试验显示，脑脊液中HTLV-III特异性IgG的百分比高于血清中的百分比，这表明神经组织的HTLV-III感染发生在大多数与AIDS或其相关复合物相关的神经系统疾病患者中。
Intra-blood-brain-barrier production of virus-specific antibody is good evidence of infection within the blood-brain barrier. Patients with the acquired immuno-deficiency syndrome (AIDS) have an increased incidence of neurologic abnormalities--i.e., unexplained, diffuse encephalopathy manifested clinically as chronic progressive dementia. To define the role of human T-cell lymphotropic virus Type III (HTLV-III), the etiologic agent of AIDS, in the pathogenesis of neurologic dysfunction, we compared cerebrospinal fluid and serum from patients with neurologic symptoms associated with AIDS and the AIDS-related complex for the presence of antibodies directed against HTLV-III. Antibodies directed against HTLV-III antigens were detected by four immunologic tests: a fixed-cell immunofluorescence assay, an enzyme-linked immunosorbent assay, immunoblots of viral lysates, and immunoprecipitation of cellular lysates. All patients were seropositive, and 22 of 23 (96 per cent) had HTLV-III-specific antibodies in their cerebrospinal fluid. Unique oligoclonal IgG bands were detected in the cerebrospinal fluid, and the rate of IgG synthesis within the blood-brain barrier was elevated. In eight of nine patients tested, the enzyme-linked immunosorbent assay showed that the percentage of HTLV-III-specific IgG in cerebrospinal fluid was higher than in serum, suggesting that HTLV-III infection of neurologic tissue occurs in the majority of patients with neurologic disease associated with AIDS or its related complex.
影响因子：
56.9
作者：
GONDA, MA;WONGSTAAL, F;GILDEN, RV
通讯作者：
GILDEN, RV
影响因子：
56.9
作者：
ALLAN, JS;COLIGAN, JE;ESSEX, M
通讯作者：
ESSEX, M