Aerobic exercise enhanced endothelium-dependent vasorelaxation in mesenteric arteries in spontaneously hypertensive rats: the role of melatonin.
Aerobic exercise enhanced endothelium-dependent vasorelaxation in mesenteric arteries in spontaneously hypertensive rats: the role of melatonin.
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有氧运动增强自发性高血压大鼠肠系膜动脉内皮依赖性血管舒张：褪黑激素的作用
DOI：
10.1038/s41440-018-0066-9
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发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Shi L
中科院分区：
文献类型：
--
作者：
Qiu F;Liu X;Zhang Y;Wu Y;Xiao D;Shi L
褪黑激素是一种主要由松果体合成的神经内分泌激素，具有多种心血管益处。规律的体力活动是预防和控制高血压的有效非药物疗法。在本研究中，我们假设褪黑激素在有氧运动诱导的自发性高血压大鼠（SHR）肠系膜动脉（MAs）内皮依赖性血管舒张增加中起着重要作用，褪黑激素受体依赖性的方式。为了验证这一假设，我们评估了血压正常的Wistar京都（WKY），SHR和在跑步机上训练8周的SHR（SHR-EX）的血管机械和功能特性。运动训练产生了显着降低血压和心率在SHR，这是显着衰减luzindole，一种非选择性褪黑素受体（MT 1/MT 2）拮抗剂的腹腔注射。SHR组血清褪黑素水平在8：00-9：00和21：00-22：00显著低于WKY组，而运动训练则缩小了这一差异。与年龄匹配的WKY相比，SHR中乙酰胆碱诱导的内皮依赖性血管舒张显著减弱。运动训练和luzindole改善这种内皮依赖性舒张功能障碍的高血压。免疫组织化学和Western印迹显示，MT 2受体和eNOS的蛋白表达，以及它们在SHR内皮细胞层中的共定位，显著降低;运动训练抑制了这种降低。这些结果提供的证据表明，定期运动对改善MA中的内皮依赖性血管舒张具有有益作用，其中褪黑激素通过作用于MT 2受体以增加NO产生和/或NO生物利用度而起关键作用。
Melatonin, a neuroendocrine hormone synthesized primarily by the pineal gland, provides various cardiovascular benefits. Regular physical activity is an effective non-pharmacological therapy for the prevention and control of hypertension. In the present study, we hypothesized that melatonin plays an important role in the aerobic exercise-induced increase of endothelium-dependent vasorelaxation in the mesenteric arteries (MAs) of spontaneously hypertensive rats (SHRs) in a melatonergic receptor-dependent manner. To test this hypothesis, we evaluated the vascular mechanical and functional properties in normotensive Wistar Kyoto (WKY), SHRs, and SHRs that were trained on a treadmill (SHR-EX) for 8 weeks. Exercise training produced a significant reduction in blood pressure and heart rate in SHR, which was significantly attenuated by the intraperitoneal administration of luzindole, a non-selective melatonin receptor (MT1/MT2) antagonist. Serum melatonin levels in the SHR group were significantly lower than those in the WKY group at 8:00–9:00 and 21:00–22:00, while exercise training reduced this difference. Endothelium-dependent vessel relaxation induced by acetylcholine was significantly blunted in SHR compared with age-matched WKY. Both exercise training and luzindole ameliorated this endothelium-dependent impairment of relaxation in hypertension. Immunohistochemistry and Western blotting showed that the protein expression of the MT2 receptor and eNOS, as well as their colocalization in the endothelial cell layer in SHRs, was significantly decreased; as exercise training suppressed this reduction. These results provide evidence that regular exercise has a beneficial effect on improving endothelium-dependent vasorelaxation in MAs, in which melatonin plays a critical role by acting on MT2 receptors to increase NO production and/or NO bioavailability.
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影响因子：
5
作者：
Doolen, S;Krause, DN;Duckles, SP
通讯作者：
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