AtHKT1 gene regulating K(+) state in whole plant improves salt tolerance in transgenic tobacco plants.
AtHKT1 gene regulating K(+) state in whole plant improves salt tolerance in transgenic tobacco plants.
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AtHKT1 基因调节全株 K 状态提高转基因烟草植物的耐盐性
DOI：
10.1038/s41598-018-34660-9
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发表时间：
2018-11-08
影响因子：
4.6
通讯作者：
Gan Y
中科院分区：
文献类型：
--
作者：
Wang L;Liu Y;Feng S;Wang Z;Zhang J;Zhang J;Wang D;Gan Y
K+的状况对植物健康至关重要。然而，很少有人知道，如果高亲和力钾转运蛋白HKT可以帮助K+的盐胁迫下的保留。本文研究了拟南芥转运蛋白基因（AtHKT1）对烟草（Nicotiana tabacum L.）K+状态、Na+诱导毒性和耐盐性的影响。以转AtHKT1基因的烟草株系（T1、T2、... T6）为材料，在全株水平和分子水平上与非转基因植株进行了比较。AtHKT1基因在转基因烟草的茎、根和叶脉的木质部中均有表达，其中T3株系表达量最高。在第15天，在200 mmol L−1 NaCl胁迫处理中，转基因植株保持健康的K+状态，而对照植株的K+含量降低了70%，叶和茎中的Na+含量是转基因株系的1.7倍。AtHKT1基因的表达增强了SOD、CAT和POD活性，提高了叶绿素和可溶性糖含量及根系活力，降低了MDA和脯氨酸含量及电解质渗漏破坏。AtHKT1的组成型过表达有助于维持健康的K+状态，同时降低Na+毒性，这可能是盐胁迫下烟草生产力最大化的一种可能机制。
The status of K+ is important for plant health. However, little is known about if high-affinity potassium transporter HKTs may help K+ retention under salt stress. Here, we determined the effect of Arabidopsis thaliana transporter gene (AtHKT1) on the K+ status, Na+-induced toxicity, and salt tolerance in tobacco (Nicotiana tabacum L.). Six AtHKT1 transformed tobacco lines (T1, T2, … T6) were contrasted with a non-transgenic plantlet at the whole-plant and molecule levels. AtHKT1 gene was expressed in the xylems of stem, root and leaf vein in the transgenic tobacco, with the line T3 having highest expression. At Day 15, in the 200 mmol L−1 NaCl stress treatment, the transgenic plants remained a healthy K+ status, while the control plants decreased K+ content by 70% and Na+ contents in leaves and stems were 1.7 times that in the transgenic line. The AtHKT1 expression enhanced the activities of SOD, CAT and POD, raised chlorophyll and soluble sugar contents and root activity, and decreased MDA and proline contents and electrolyte leakage destruction. The constitutive over-expression of AtHKT1 that helps maintain a healthy K+ status while reducing Na+ toxicity may serve as a possible mechanism in maximizing productivity of tobacco under salt stress.
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影响因子：
6.9
作者：
Ardie SW;Xie L;Takahashi R;Liu S;Takano T
通讯作者：
Takano T
影响因子：
4.6
作者：
Bharti N;Pandey SS;Barnawal D;Patel VK;Kalra A
通讯作者：
Kalra A
影响因子：
5.6
作者：
Kumar S;Beena AS;Awana M;Singh A
通讯作者：
Singh A
DOI：
10.1073/pnas.082123799
发表时间：
2002-04-30
影响因子：
11.1
作者：
Mäser, P;Hosoo, Y;Uozumi, N
通讯作者：
Uozumi, N
影响因子：
7.2
作者：
Byrt, Caitlin Siobhan;Xu, Bo;Gilliham, Matthew
通讯作者：
Gilliham, Matthew