Molecular Mechanisms Underlying Hull-Caryopsis Adhesion/Separation Revealed by Comparative Transcriptomic Analysis of Covered/Naked Barley (Hordeum vulgare L.).
Molecular Mechanisms Underlying Hull-Caryopsis Adhesion/Separation Revealed by Comparative Transcriptomic Analysis of Covered/Naked Barley (Hordeum vulgare L.).
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通过覆盖/裸大麦（Hordeum vulgare L.）的比较转录组分析揭示了壳-颖果粘附/分离的分子机制。
DOI：
10.3390/ijms160614181
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发表时间：
2015-06-23
影响因子：
5.6
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Duan R;Xiong H;Wang A;Chen G
大麦的被覆盖/裸露颖果性状是一个重要的农艺性状，因为它直接与饮食用途有关。覆盖/裸露颖果的形成是由NUD转录因子控制的，该转录因子参与果皮角质层的发育。然而，这一特征的分子机制至今仍不清楚。本研究利用RNA-seq技术对西藏青稞地方品种独粒黄和盖麦Morex花后3周籽粒的比较转录组进行了分析。共鉴定出4031个差异表达基因。Nud基因在Morex中过表达，在独粒黄中微量表达。在17个角质层相关DEG中，16个在Morex中下调，1个上调。这些结果表明，覆被颖果中的Nud基因可能下调了角质层相关基因，这可能导致果皮上的渗透性角质层，导致壳-颖果器官融合。裸露颖果果皮上的功能性角质层可能是Nud基因缺失或低表达的结果。裸大麦的功能角质层为颖果与船体的分离提供了一个完美的边界。
The covered/naked caryopsis trait of barley is an important agronomic trait because it is directly linked to dietary use. The formation of covered/naked caryopsis is controlled by an NUD transcription factor, which is involved in pericarp cuticle development. However, the molecular mechanism underlying this trait remains so far largely unknown. In this study, comparative transcriptomes of grains three weeks after anthesis of Tibetan Hulless barley landrace Dulihuang and covered barley Morex were analyzed using RNA-seq technique. A total of 4031 differentially expressed genes (DEGs) were identified. The Nud gene was overexpressed in Morex, with trace expression in Dulihuang. Among seventeen cuticle related DEGs, sixteen were down regulated and one up regulated in Morex. These results suggest that the Nud gene in covered caryopsis might down regulate cuticle related genes, which may cause a permeable cuticle over pericarp, leading to a hull-caryopsis organ fusion. A functional cuticle covering the pericarp of naked caryopsis might be the result of deletion or low expression level of the Nud gene. The functional cuticle defines a perfect boundary to separate the caryopsis from the hull in naked barley.
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影响因子：
4.2
作者：
Pourkheirandish M;Komatsuda T
通讯作者：
Komatsuda T
影响因子：
11.6
作者：
Sieber, P;Schorderet, M;Nawrath, C
通讯作者：
Nawrath, C
DOI：
10.1199/tab.0167
发表时间：
2013-12-27
期刊：
The arabidopsis book
影响因子：
--
作者：
Nawrath, Christiane;Schreiber, Lukas;Kunst, Ljerka
通讯作者：
Kunst, Ljerka
影响因子：
2.6
作者：
FREEMAN, PL;PALMER, GH
通讯作者：
PALMER, GH
影响因子：
--
作者：
Newman, CW;Newman, RK
通讯作者：
Newman, RK