Analysis of drug interactions with serum proteins and related binding agents by affinity capillary electrophoresis: A review.
Analysis of drug interactions with serum proteins and related binding agents by affinity capillary electrophoresis: A review.
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DOI：
10.1002/elps.202200191
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发表时间：
2022-12
期刊：
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
--
作者：
血清蛋白等生物分子可与体内药物相互作用，影响药物的药效。分析这些相互作用的具体和准确的方法对于药物开发或监测以及诊断目的至关重要。亲和毛细管电泳法(ACE)是一种可用于检测药物与血清蛋白或血清或血液中发现的其他试剂之间的结合的技术。本文将回顾血管紧张素转换酶的基本原理，以及相关的基于亲和力的毛细管电泳法，并综述该领域在药物-蛋白质相互作用表征方面的最新进展。将概述ACE和CE中可用于这类工作的各种格式，包括每种方法的相对优势或劣势。还将介绍ACE和基于亲和力的CE方法在分析药物与血清蛋白和其他结合剂相互作用方面的各种应用。将讨论的ACE和相关技术的应用包括药物与血清试剂的相互作用研究、使用血清蛋白质的手性药物分离，以及在杂交方法中使用CE来表征药物与血清蛋白质的结合。
Biomolecules such as serum proteins can interact with drugs in the body and influence their pharmaceutical effects. Specific and precise methods that analyze these interactions are critical for drug development or monitoring and for diagnostic purposes. Affinity capillary electrophoresis (ACE) is one technique that can be used to examine the binding between drugs and serum proteins, or other agents found in serum or blood. This article will review the basic principles of ACE, along with related affinity‐based capillary electrophoresis (CE) methods, and examine recent developments that have occurred in this field as related to the characterization of drug–protein interactions. An overview will be given of the various formats that can be used in ACE and CE for such work, including the relative advantages or weaknesses of each approach. Various applications of ACE and affinity‐based CE methods for the analysis of drug interactions with serum proteins and other binding agents will also be presented. Applications of ACE and related techniques that will be discussed include drug interaction studies with serum agents, chiral drug separations employing serum proteins, and the use of CE in hybrid methods to characterize drug binding with serum proteins.
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DOI：
10.1016/j.cca.2013.07.013
发表时间：
2013-10-21
期刊：
Clinica chimica acta; international journal of clinical chemistry
影响因子：
--
作者：
Anguizola J;Matsuda R;Barnaby OS;Hoy KS;Wa C;DeBolt E;Koke M;Hage DS
通讯作者：
Hage DS
影响因子：
7.4
作者：
Berezovski, M;Krylov, SN
通讯作者：
Krylov, SN
DOI：
10.1016/j.jchromb.2012.11.007
发表时间：
2012-12-12
影响因子：
3
作者：
Abd El-Hady, D.;Albishri, H. M.
通讯作者：
Albishri, H. M.
影响因子：
7.4
作者：
Amini, A;Westerlund, D
通讯作者：
Westerlund, D
影响因子：
2.9
作者：
Andreev, VP;Pliss, NS;Righetti, PG
通讯作者：
Righetti, PG