Structure of cyanobacterial phycobilisome core revealed by structural modeling and chemical cross-linking.
Structure of cyanobacterial phycobilisome core revealed by structural modeling and chemical cross-linking.
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DOI：
10.1126/sciadv.aba5743
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发表时间：
2021-01
期刊：
影响因子：
13.6
通讯作者：
Blankenship RE
中科院分区：
文献类型：
--
作者：
Liu H;Zhang MM;Weisz DA;Cheng M;Pakrasi HB;Blankenship RE
蓝藻捕光复合体具有高效的能量捕获、传输和调节反应中心的功能。在蓝藻和红藻中，指示从藻胆体（PBS）天线复合体的反应中心的有效激发能量转移的结构基础仍然不清楚。PBS有几个外围杆和一个结合到类囊体膜的中心核心，允许与光系统II（PSII）和PSI的能量耦合。在这里，我们结合化学交联质谱同源建模，提出了一个三环藻蓝藻PBS核心结构。我们的模型揭示了一个侧视图交叉配置的两个基本的圆柱体，巩固的ApcE PB环和ApcD，这有利于能量转移到PSII和PSI，分别组成的锚定域的重要作用。PBS芯的不平坦底表面与PSII的平坦还原侧形成对比。两个基础圆柱体和PSII之间的额外空间为调节元件提供了增加的可达性，橙子类胡萝卜素蛋白，其是调节光化学活性所必需的。
Cyanobacterial light-harvesting complex features efficient energy capture, transmission, and regulation to reaction centers. In cyanobacteria and red algae, the structural basis dictating efficient excitation energy transfer from the phycobilisome (PBS) antenna complex to the reaction centers remains unclear. The PBS has several peripheral rods and a central core that binds to the thylakoid membrane, allowing energy coupling with photosystem II (PSII) and PSI. Here, we have combined chemical cross-linking mass spectrometry with homology modeling to propose a tricylindrical cyanobacterial PBS core structure. Our model reveals a side-view crossover configuration of the two basal cylinders, consolidating the essential roles of the anchoring domains composed of the ApcE PB loop and ApcD, which facilitate the energy transfer to PSII and PSI, respectively. The uneven bottom surface of the PBS core contrasts with the flat reducing side of PSII. The extra space between two basal cylinders and PSII provides increased accessibility for regulatory elements, e.g., orange carotenoid protein, which are required for modulating photochemical activity.
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影响因子：
4.3
作者：
Calzadilla, Pablo I.;Muzzopappa, Fernando;Kirilovsky, Diana
通讯作者：
Kirilovsky, Diana
影响因子：
4.3
作者：
GINDT, YM;ZHOU, JH;SAUER, K
通讯作者：
SAUER, K
DOI：
10.1111/j.1432-1033.1992.tb16857.x
发表时间：
1992-05-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
GLAUSER, M;BRYANT, DA;ZUBER, H
通讯作者：
ZUBER, H
影响因子：
4.3
作者：
Marx, Ailie;Adir, Noam
通讯作者：
Adir, Noam
影响因子：
6.4
作者：
Liu, Haijun;Weisz, Daniel A.;Blankenship, Robert E.
通讯作者：
Blankenship, Robert E.